Abstract
The bioconversion of linoleic acid (LA) to conjugated linoleic acid (CLA) was investigated to examine LA-adaptation of Bifidobacterium breve KCTC 3461 to additions of 1 to 5 mg/mL of LA overtime. To induce LA-adaptation, B. breve KCTC 3461 was treated with LA, according to three schemes. For LA-adapted B. breve the maximum concentration of CLA, 300-350 μg/mL, was obtained in cys-MRS medium containing 1 mg/mL of LA. The CLA production significantly increased with increasing LA concentration, from 1 to 4 mg/mL, but the conversion of LA to CLA gradually decreased. The CLA production capability of B. breve, and its tolerance, improved significantly with LA-adaptation. The addition of LA (1 mg/mL) into the culture broth after 24 h of cultivation in a 100-mL media bottle was most effective at promoting CLA production. In a 2.5-L stirred-tank bioreactor, the observed conversion and productivity of 56.6% and 35.4 μgmL-1h-1, respectively, by LA-adapted B. breve were approximately 6.6 and 9.8 times higher than those of LA-unadapted B. breve.
| Original language | English |
|---|---|
| Pages (from-to) | 357-361 |
| Number of pages | 5 |
| Journal | Biotechnology and Bioprocess Engineering |
| Volume | 10 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2005 |
Bibliographical note
Funding Information:^ÅâåçïäÉÇÖÉãÉåíë This study was supported by research grants from the Korea Science and Engineering Foundation (KOSEF) through the Applied Rheology Center (ARC), an official KOSEF-created engineering research center (ERC) at Korea University, Seoul, Korea.
Keywords
- Bifidobacterium breve
- Bioconversion
- Conjugated linoleic acid (CLA)
- Linoleic acid (LA)
- Linoleic acid-adaptation
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Applied Microbiology and Biotechnology
- Biomedical Engineering