Bioconversion of linoleic acid to conjugated linoleic acid by Bifidobacterium breve

Yoon Seok Song, Seong Woo Kang, Deok Kun Oh, Yong Taik Rho, Suk In Hong, Seung Wook Kim

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17 Citations (Scopus)


The bioconversion of linoleic acid (LA) to conjugated linoleic acid (CLA) was investigated to examine LA-adaptation of Bifidobacterium breve KCTC 3461 to additions of 1 to 5 mg/mL of LA overtime. To induce LA-adaptation, B. breve KCTC 3461 was treated with LA, according to three schemes. For LA-adapted B. breve the maximum concentration of CLA, 300-350 μg/mL, was obtained in cys-MRS medium containing 1 mg/mL of LA. The CLA production significantly increased with increasing LA concentration, from 1 to 4 mg/mL, but the conversion of LA to CLA gradually decreased. The CLA production capability of B. breve, and its tolerance, improved significantly with LA-adaptation. The addition of LA (1 mg/mL) into the culture broth after 24 h of cultivation in a 100-mL media bottle was most effective at promoting CLA production. In a 2.5-L stirred-tank bioreactor, the observed conversion and productivity of 56.6% and 35.4 μgmL-1h-1, respectively, by LA-adapted B. breve were approximately 6.6 and 9.8 times higher than those of LA-unadapted B. breve.

Original languageEnglish
Pages (from-to)357-361
Number of pages5
JournalBiotechnology and Bioprocess Engineering
Issue number4
Publication statusPublished - 2005

Bibliographical note

Funding Information:
^ÅâåçïäÉÇÖÉãÉåíë This study was supported by research grants from the Korea Science and Engineering Foundation (KOSEF) through the Applied Rheology Center (ARC), an official KOSEF-created engineering research center (ERC) at Korea University, Seoul, Korea.


  • Bifidobacterium breve
  • Bioconversion
  • Conjugated linoleic acid (CLA)
  • Linoleic acid (LA)
  • Linoleic acid-adaptation

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Biomedical Engineering


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