Biodegradation of 2,4,5-trichlorophenol using cell-free culture broths of Phanerochaete chrysosporium

S. H. Choi, S. H. Moon, J. S. Lee, M. B. Gu

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)


Cell-free culture broth of Phanerochaete chrysosporium has been adopted to biologically degrade 2,4,5-trichlorophenol. Two different medium compositions of nitrogen-sufficient and nitrogen-limited were compared for their distribution of isozymes, activity of lignin peroxidase, and production of oxalate. The two different culture broths were tested for their ability to degrade 2,4,5-trichlorophenol, and the biodegradation efficiency was estimated in terms of the disappearance of 2,4,5-trichlorophenol. The degradation efficiency for the nitrogen-limited culture broth was higher than that of the nitrogen-sufficient culture broth, since the nitrogen-limited culture broth induced lignin peroxidases (LiPs) and manganese peroxidases (MnPs), and contained sufficient oxalate for producing necessary radicals. Finally, the possible mechanism of 2,4,5-CP degradation using the nitrogen-limited culture broth was proposed.

Original languageEnglish
Pages (from-to)759-763
Number of pages5
JournalJournal of microbiology and biotechnology
Issue number6
Publication statusPublished - 2000
Externally publishedYes


  • 2,4,5-trichlorophenol
  • Cell-free culture broth
  • Lignin peroxidase
  • Oxalate
  • Phanerochaete chrysosporium

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology


Dive into the research topics of 'Biodegradation of 2,4,5-trichlorophenol using cell-free culture broths of Phanerochaete chrysosporium'. Together they form a unique fingerprint.

Cite this