Biodiesel production by enzymatic process using Jatropha oil and waste soybean oil

Ja Hyun Lee, Sung Bong Kim, Hah Young Yoo, Young Joon Suh, Gyung Bo Kang, Woo In Jang, Jongwon Kang, Chulhwan Park, Seung Wook Kim

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)


In this study, non-edible Jatropha oil and postcooking waste soybean oil were utilized for enzymatic biodiesel production. The process was optimized by using a statistical method. In addition, a novel continuous process using co-immobilized Rhizopus oryzae and Candida rugosa lipases was developed. The optimum conditions for the batch process were determined to be a reaction temperature of 45oC, an agitation speed of 250 rpm, 10 wt% of water, and 20% of immobilized lipases. A conversion of about 98% at 4 h could be achieved for biodiesel production using Jatropha oil, while a conversion of about 97% at 4 h was achieved from waste soybean oil. A packed bed reactor charged with co-immobilized lipases was employed for continuous biodiesel production from Jatropha and waste soybean oil. The reactor consisted of a jacketed glass column (ID 25 mm × 130 mm), in which a temperature of 45 C was maintained by water circulation. A maximum conversion of about 80% in 24 h at a flow rate of 0.8 mL/ min was achieved with the continuous process, whereas in the two-stage continuous process, a conversion of about 90% in 72 h was attained at a flow rate of 0.1 mL/min.

Original languageEnglish
Pages (from-to)703-708
Number of pages6
JournalBiotechnology and Bioprocess Engineering
Issue number4
Publication statusPublished - 2013 Jul

Bibliographical note

Funding Information:
This work was supported by the Advanced Biomass R&D Center (ABC-2011-0031360) of the Global Frontier Project funded by the Ministry of Education, Science and Technology along with the Honam Petrochemical Corporation.


  • biodiesel
  • continuous process
  • lipase
  • optimization
  • response surface methodology

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Biomedical Engineering


Dive into the research topics of 'Biodiesel production by enzymatic process using Jatropha oil and waste soybean oil'. Together they form a unique fingerprint.

Cite this