Biodiesel production by lipases co-immobilized on the functionalized activated carbon

Ja Hyun Lee, Ju Hun Lee, Dong Sup Kim, Hah Young Yoo, Chulhwan Park, Seung Wook Kim

Research output: Contribution to journalArticlepeer-review

53 Citations (Scopus)


Enzymatic conversion of biodiesel was performed by using co-immobilized lipases. The surface modification of activated carbon (AC) was carried out to improve the stability and reusability of enzyme support. The surface modified AC (ACO: Activated carbon oxide) clearly showed porous and rugged surfaces by scanning electron microscope (SEM) analysis. Subsequently, the Fourier-transform infrared spectroscopy (FT-IR) and X-ray photoelectron spectroscopy (XPS) were carried out to evaluate the generation of functional groups. The highest activity of immobilized lipase was about 10,360 U/g matrix under pretreatment with co-immobilization method. The activity was maintained about 80% after 20 times of reuse in the batch system. The immobilized lipases were applied to the biodiesel production from different sources of oils such as soybean oil, waste cooking oil and algal oil. The maximum biodiesel conversions from soybean oil, waste cooking oil, and algal oil were found to be 98.5%, 95.7%, and 93.8%, respectively, within 4 h.

Original languageEnglish
Article number100248
JournalBioresource Technology Reports
Publication statusPublished - 2019 Sept

Bibliographical note

Publisher Copyright:
© 2019

Copyright 2021 Elsevier B.V., All rights reserved.


  • Activated carbon
  • Algal oil
  • Biodiesel
  • Immobilization
  • Lipase

ASJC Scopus subject areas

  • Bioengineering
  • Renewable Energy, Sustainability and the Environment
  • Environmental Engineering
  • Waste Management and Disposal


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