TY - JOUR
T1 - Biofilm development of Bacillus siamensis ATKU1 on pristine short chain low-density polyethylene
T2 - A case study on microbe-microplastics interaction
AU - Tarafdar, Abhrajyoti
AU - Lee, Jae Ung
AU - Jeong, Ji Eun
AU - Lee, Hanbyul
AU - Jung, Yerin
AU - Oh, Han Bin
AU - Woo, Han Young
AU - Kwon, Jung Hwan
N1 - Funding Information:
This study was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MEST) (no. 2020R1A2C2009244 ). H.Y.W. is grateful for the financial support from the National Research Foundation (NRF) of Korea ( NRF-2019R1A6A1A11044070 ). Authors are thankful to Ms. Yeonjae Yoo, Division of Environmental Science and Ecological Engineering, Korea University, for her valuable suggestions regarding phylogenetic tree construction. The authors also thank Editage ( www.editage.co.kr ) for providing language editing guidance and Park Systems for their kind instrumental assistance.
Publisher Copyright:
© 2020 Elsevier B.V.
PY - 2021/5/5
Y1 - 2021/5/5
N2 - A low-density polyethylene (LDPE) degrading bacterial strain (ATKU1) was isolated (99.86% similar with Bacillus siamensis KCTC 13613T) from a plastic dumping site to study interactions between microplastics (< 5 mm) and microorganisms. The strain was found (by scanning electron microscopy) to form biofilm on the microplastic surface after its interaction with LDPE (avg. Mw~4,000 Da and avg. Mn~1,700 Da) as a sole carbon source. Atomic force microscopy (AFM) showed the biofilm's 3-D developmental patterns and significantly increased Young's modulus of the LDPE surface after microbial treatment. Most of the viable bacteria attached to biofilms rather than media, which suggested their ability to utilize LDPE. Absorption bands of carbonyl, alkenyl, acyl, ester, primary-secondary alcohol, alkene groups and nitric oxides were found on the treated LDPE particles using Fourier-transform infrared spectroscopy. Fourier transform-ion cyclotron resonance mass spectrometry of the media indicated compositional shifts of the compounds after treatment (i.e., increase in the degree of unsaturation and increment in oxygen-to-carbon ratio) and presence of unsaturated hydrocarbons, polyketides, terpenoids, aliphatic/peptides, dicarboxylic acids, lipid-like compounds were hinted. The plastic degrading abilities of Bacillus siamensis ATKU1 suggest its probable application for large scale plastic bioremediation facility.
AB - A low-density polyethylene (LDPE) degrading bacterial strain (ATKU1) was isolated (99.86% similar with Bacillus siamensis KCTC 13613T) from a plastic dumping site to study interactions between microplastics (< 5 mm) and microorganisms. The strain was found (by scanning electron microscopy) to form biofilm on the microplastic surface after its interaction with LDPE (avg. Mw~4,000 Da and avg. Mn~1,700 Da) as a sole carbon source. Atomic force microscopy (AFM) showed the biofilm's 3-D developmental patterns and significantly increased Young's modulus of the LDPE surface after microbial treatment. Most of the viable bacteria attached to biofilms rather than media, which suggested their ability to utilize LDPE. Absorption bands of carbonyl, alkenyl, acyl, ester, primary-secondary alcohol, alkene groups and nitric oxides were found on the treated LDPE particles using Fourier-transform infrared spectroscopy. Fourier transform-ion cyclotron resonance mass spectrometry of the media indicated compositional shifts of the compounds after treatment (i.e., increase in the degree of unsaturation and increment in oxygen-to-carbon ratio) and presence of unsaturated hydrocarbons, polyketides, terpenoids, aliphatic/peptides, dicarboxylic acids, lipid-like compounds were hinted. The plastic degrading abilities of Bacillus siamensis ATKU1 suggest its probable application for large scale plastic bioremediation facility.
KW - Biodegradation
KW - Biofilm
KW - LDPE
KW - Microplastics
KW - Van Krevelen
UR - http://www.scopus.com/inward/record.url?scp=85097157176&partnerID=8YFLogxK
U2 - 10.1016/j.jhazmat.2020.124516
DO - 10.1016/j.jhazmat.2020.124516
M3 - Article
C2 - 33243655
AN - SCOPUS:85097157176
SN - 0304-3894
VL - 409
JO - Journal of hazardous materials
JF - Journal of hazardous materials
M1 - 124516
ER -