TY - JOUR
T1 - Biofilm reduction potential of micro-plasma discharged water (m-PDW) against the microbes isolated from a tofu manufacturing plant
AU - Kim, Mi Hyun
AU - Khan, Muhammad Saiful Islam
AU - Lee, Kwang Won
AU - Kim, Yun Ji
N1 - Funding Information:
This research was supported by the main Research Program ( E0142103 ) of Korea Food Research Institute funded by the Ministry of Science, ICT and Future Planning .
Funding Information:
This research was supported by the main Research Program (E0142103) of Korea Food Research Institute funded by the Ministry of Science, ICT and Future Planning.
Publisher Copyright:
© 2018 Elsevier Ltd
PY - 2018/11
Y1 - 2018/11
N2 - In this study, biofilm inactivation potential of micro-plasma discharged water (m-PDW), a commercially available water purifier known as EP purification, was evaluated. Bacterial strains were collected from the various contaminated area of tofu manufacturing industry, overall 15 different types of bacterial strains were isolated and their biofilm formation ability was determined by using crystal violet (CV) staining. Three different strains (20% of the isolated strains), namely, Brevundimonas naejangsanensis, Delfita lacustris and Lactococcus lactis, with biofilm density (OD590) > 0.4, were identified as biofilm-producing strains. These three biofilm-producing strains were used to form biofilm on stainless steel (SS) coupons in a laboratory condition for four days and the biofilm containing coupons were treated with EP purification device. The inactivation obtained after 40 min of treatment was 4.8 and 4.2 log CFU/coupon for Brevundimonas naejangsanensis and Delfita lacustris, respectively, whereas no viable count was detected for Lactococcus lactis after the same treatment time. Biofilm reduction effects of m-PDW treatment differ according to biofilm-producing ability or strain characteristics. SEI (Secondary Electron Images) was obtained by field emission scanning electron microscope (FE-SEM) that showed m-PDW treatment has significant reduction potential on biofilm inactivation for the sample collected from tofu manufacturing industry.
AB - In this study, biofilm inactivation potential of micro-plasma discharged water (m-PDW), a commercially available water purifier known as EP purification, was evaluated. Bacterial strains were collected from the various contaminated area of tofu manufacturing industry, overall 15 different types of bacterial strains were isolated and their biofilm formation ability was determined by using crystal violet (CV) staining. Three different strains (20% of the isolated strains), namely, Brevundimonas naejangsanensis, Delfita lacustris and Lactococcus lactis, with biofilm density (OD590) > 0.4, were identified as biofilm-producing strains. These three biofilm-producing strains were used to form biofilm on stainless steel (SS) coupons in a laboratory condition for four days and the biofilm containing coupons were treated with EP purification device. The inactivation obtained after 40 min of treatment was 4.8 and 4.2 log CFU/coupon for Brevundimonas naejangsanensis and Delfita lacustris, respectively, whereas no viable count was detected for Lactococcus lactis after the same treatment time. Biofilm reduction effects of m-PDW treatment differ according to biofilm-producing ability or strain characteristics. SEI (Secondary Electron Images) was obtained by field emission scanning electron microscope (FE-SEM) that showed m-PDW treatment has significant reduction potential on biofilm inactivation for the sample collected from tofu manufacturing industry.
KW - Biofilm
KW - EP purification
KW - Stainless steel (SS) coupon
KW - Tofu
KW - m-PDW
UR - http://www.scopus.com/inward/record.url?scp=85051113569&partnerID=8YFLogxK
U2 - 10.1016/j.lwt.2018.07.047
DO - 10.1016/j.lwt.2018.07.047
M3 - Article
AN - SCOPUS:85051113569
SN - 0023-6438
VL - 97
SP - 719
EP - 724
JO - LWT
JF - LWT
ER -