Biokinetic parameter estimation for degradation of 2,4,6-trinitrotoluene (TNT) with Pseudomonas putida KP-T201

Chulhwan Park; Tak Hyun Kim; Sangyong Kim; Jinwon Lee; Seung Wook Kim

doi:10.1263/jbb.94.57

Biokinetic parameter estimation for degradation of 2,4,6-trinitrotoluene (TNT) with Pseudomonas putida KP-T201

Chulhwan Park, Tak Hyun Kim, Sangyong Kim, Jinwon Lee, Seung Wook Kim

Research output: Contribution to journal › Article › peer-review

28 Citations (Scopus)

Abstract

The purpose of this study is to determine the biodegradation kinetics of 2,4,6-trinitrotoluene (TNT) by a newly isolated microorganism. Hundreds of microorganisms were isolated from explosives-contaminated soil in Korea. Examination of culture tests revealed that a few species of microorganisms have good ability to degrade TNT. The most efficient one was selected and identified as Pseudomonasputida KP-T201. Biodegradation of TNT was tested in a batch reactor using a pure culture of P. putida KP-T201. The effect of different concentrations of TNT on the rate of bacterial biodegradation was investigated. The Haldane equation seems to be an adequate expression for the cell growth data, and the kinetic constants obtained were μ_m=0.65 h^-1, K_s=0.62 mgl^-1, and K_i=115 mgl^-1. The dependence of the bacterial specific growth rate on the concentration of TNT could be explained as a conventional model of substrate inhibition.

Original language	English
Pages (from-to)	57-61
Number of pages	5
Journal	Journal of Bioscience and Bioengineering
Volume	94
Issue number	1
DOIs	https://doi.org/10.1263/jbb.94.57
Publication status	Published - 2002

Bibliographical note

Funding Information:
This work was supportedb y MOCIE (Korea Ministry of Commerce,I ndustry and Energy) and MOENV (Korea Ministry of Environment).

Keywords

Biodegradation
Haldane equation
Inhibition
Pseudomonas putida
TNT

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1263/jbb.94.57

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title = "Biokinetic parameter estimation for degradation of 2,4,6-trinitrotoluene (TNT) with Pseudomonas putida KP-T201",

abstract = "The purpose of this study is to determine the biodegradation kinetics of 2,4,6-trinitrotoluene (TNT) by a newly isolated microorganism. Hundreds of microorganisms were isolated from explosives-contaminated soil in Korea. Examination of culture tests revealed that a few species of microorganisms have good ability to degrade TNT. The most efficient one was selected and identified as Pseudomonasputida KP-T201. Biodegradation of TNT was tested in a batch reactor using a pure culture of P. putida KP-T201. The effect of different concentrations of TNT on the rate of bacterial biodegradation was investigated. The Haldane equation seems to be an adequate expression for the cell growth data, and the kinetic constants obtained were μm=0.65 h-1, Ks=0.62 mgl-1, and Ki=115 mgl-1. The dependence of the bacterial specific growth rate on the concentration of TNT could be explained as a conventional model of substrate inhibition.",

keywords = "Biodegradation, Haldane equation, Inhibition, Pseudomonas putida, TNT",

author = "Chulhwan Park and Kim, {Tak Hyun} and Sangyong Kim and Jinwon Lee and Kim, {Seung Wook}",

note = "Funding Information: This work was supportedb y MOCIE (Korea Ministry of Commerce,I ndustry and Energy) and MOENV (Korea Ministry of Environment).",

year = "2002",

doi = "10.1263/jbb.94.57",

language = "English",

volume = "94",

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journal = "Journal of Bioscience and Bioengineering",

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AU - Park, Chulhwan

AU - Kim, Tak Hyun

AU - Kim, Sangyong

AU - Lee, Jinwon

AU - Kim, Seung Wook

N1 - Funding Information: This work was supportedb y MOCIE (Korea Ministry of Commerce,I ndustry and Energy) and MOENV (Korea Ministry of Environment).

PY - 2002

Y1 - 2002

N2 - The purpose of this study is to determine the biodegradation kinetics of 2,4,6-trinitrotoluene (TNT) by a newly isolated microorganism. Hundreds of microorganisms were isolated from explosives-contaminated soil in Korea. Examination of culture tests revealed that a few species of microorganisms have good ability to degrade TNT. The most efficient one was selected and identified as Pseudomonasputida KP-T201. Biodegradation of TNT was tested in a batch reactor using a pure culture of P. putida KP-T201. The effect of different concentrations of TNT on the rate of bacterial biodegradation was investigated. The Haldane equation seems to be an adequate expression for the cell growth data, and the kinetic constants obtained were μm=0.65 h-1, Ks=0.62 mgl-1, and Ki=115 mgl-1. The dependence of the bacterial specific growth rate on the concentration of TNT could be explained as a conventional model of substrate inhibition.

AB - The purpose of this study is to determine the biodegradation kinetics of 2,4,6-trinitrotoluene (TNT) by a newly isolated microorganism. Hundreds of microorganisms were isolated from explosives-contaminated soil in Korea. Examination of culture tests revealed that a few species of microorganisms have good ability to degrade TNT. The most efficient one was selected and identified as Pseudomonasputida KP-T201. Biodegradation of TNT was tested in a batch reactor using a pure culture of P. putida KP-T201. The effect of different concentrations of TNT on the rate of bacterial biodegradation was investigated. The Haldane equation seems to be an adequate expression for the cell growth data, and the kinetic constants obtained were μm=0.65 h-1, Ks=0.62 mgl-1, and Ki=115 mgl-1. The dependence of the bacterial specific growth rate on the concentration of TNT could be explained as a conventional model of substrate inhibition.

KW - Biodegradation

KW - Haldane equation

KW - Inhibition

KW - Pseudomonas putida

KW - TNT

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Biokinetic parameter estimation for degradation of 2,4,6-trinitrotoluene (TNT) with Pseudomonas putida KP-T201

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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