Biological Effect of Gamma Rays According to Exposure Time on Germination and Plant Growth in Wheat

Min Jeong Hong; Dae Yeon Kim; Yeong Deuk Jo; Hong Il Choi; Joon Woo Ahn; Soon Jae Kwon; Sang Hoon Kim; Yong Weon Seo; Jin Baek Kim

doi:10.3390/app12063208

Biological Effect of Gamma Rays According to Exposure Time on Germination and Plant Growth in Wheat

Min Jeong Hong, Dae Yeon Kim, Yeong Deuk Jo, Hong Il Choi, Joon Woo Ahn, Soon Jae Kwon, Sang Hoon Kim, Yong Weon Seo, Jin Baek Kim

Research output: Contribution to journal › Article › peer-review

37 Citations (Scopus)

Abstract

Gamma rays as a type of ionizing radiation constitute a physical mutagen that induces mutations and could be effectively used in plant breeding. To compare the effects of gamma and ionizing irradiation according to exposure time in common wheat (Keumgang, IT 213100), seeds were exposed to⁶⁰ Co gamma rays at different dose rates. To evaluate the amount of free radical content, we used electron spin resonance spectroscopy. Significantly more free radicals were generated in the case of long-term compared with short-term gamma-ray exposure at the same dose of radiation. Under short-term exposure, shoot and root lengths were slightly reduced compared with those of the controls, whereas long-term exposure caused severe growth inhibition. The expression of antioxidant-related and DNA-repair-related genes was significantly decreased under long-term gamma-ray exposure. Long-term exposure caused higher radiosensitivity than short-term exposure. The results of this study could help plant breeders select an effective mutagenic induction dose rate in wheat.

Original language	English
Article number	3208
Journal	Applied Sciences (Switzerland)
Volume	12
Issue number	6
DOIs	https://doi.org/10.3390/app12063208
Publication status	Published - 2022 Mar 2

Bibliographical note

Funding Information:
Funding: This research was supported by grants from the Nuclear R&D Program of the Ministry of Science and ICT (MSIT) and the research program of KAERI, Republic of Korea.

Publisher Copyright:
© 2022 by the authors. Licensee MDPI, Basel, Switzerland.

Keywords

exposure time
free radicals
gamma rays
growth inhibition
mutation breeding
wheat

ASJC Scopus subject areas

General Materials Science
Instrumentation
General Engineering
Process Chemistry and Technology
Computer Science Applications
Fluid Flow and Transfer Processes

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10.3390/app12063208

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title = "Biological Effect of Gamma Rays According to Exposure Time on Germination and Plant Growth in Wheat",

abstract = "Gamma rays as a type of ionizing radiation constitute a physical mutagen that induces mutations and could be effectively used in plant breeding. To compare the effects of gamma and ionizing irradiation according to exposure time in common wheat (Keumgang, IT 213100), seeds were exposed to60 Co gamma rays at different dose rates. To evaluate the amount of free radical content, we used electron spin resonance spectroscopy. Significantly more free radicals were generated in the case of long-term compared with short-term gamma-ray exposure at the same dose of radiation. Under short-term exposure, shoot and root lengths were slightly reduced compared with those of the controls, whereas long-term exposure caused severe growth inhibition. The expression of antioxidant-related and DNA-repair-related genes was significantly decreased under long-term gamma-ray exposure. Long-term exposure caused higher radiosensitivity than short-term exposure. The results of this study could help plant breeders select an effective mutagenic induction dose rate in wheat.",

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note = "Funding Information: Funding: This research was supported by grants from the Nuclear R&D Program of the Ministry of Science and ICT (MSIT) and the research program of KAERI, Republic of Korea. Publisher Copyright: {\textcopyright} 2022 by the authors. Licensee MDPI, Basel, Switzerland.",

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AU - Hong, Min Jeong

AU - Kim, Dae Yeon

AU - Jo, Yeong Deuk

AU - Choi, Hong Il

AU - Ahn, Joon Woo

AU - Kwon, Soon Jae

AU - Kim, Sang Hoon

AU - Seo, Yong Weon

AU - Kim, Jin Baek

N1 - Funding Information: Funding: This research was supported by grants from the Nuclear R&D Program of the Ministry of Science and ICT (MSIT) and the research program of KAERI, Republic of Korea. Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2022/3/2

Y1 - 2022/3/2

N2 - Gamma rays as a type of ionizing radiation constitute a physical mutagen that induces mutations and could be effectively used in plant breeding. To compare the effects of gamma and ionizing irradiation according to exposure time in common wheat (Keumgang, IT 213100), seeds were exposed to60 Co gamma rays at different dose rates. To evaluate the amount of free radical content, we used electron spin resonance spectroscopy. Significantly more free radicals were generated in the case of long-term compared with short-term gamma-ray exposure at the same dose of radiation. Under short-term exposure, shoot and root lengths were slightly reduced compared with those of the controls, whereas long-term exposure caused severe growth inhibition. The expression of antioxidant-related and DNA-repair-related genes was significantly decreased under long-term gamma-ray exposure. Long-term exposure caused higher radiosensitivity than short-term exposure. The results of this study could help plant breeders select an effective mutagenic induction dose rate in wheat.

AB - Gamma rays as a type of ionizing radiation constitute a physical mutagen that induces mutations and could be effectively used in plant breeding. To compare the effects of gamma and ionizing irradiation according to exposure time in common wheat (Keumgang, IT 213100), seeds were exposed to60 Co gamma rays at different dose rates. To evaluate the amount of free radical content, we used electron spin resonance spectroscopy. Significantly more free radicals were generated in the case of long-term compared with short-term gamma-ray exposure at the same dose of radiation. Under short-term exposure, shoot and root lengths were slightly reduced compared with those of the controls, whereas long-term exposure caused severe growth inhibition. The expression of antioxidant-related and DNA-repair-related genes was significantly decreased under long-term gamma-ray exposure. Long-term exposure caused higher radiosensitivity than short-term exposure. The results of this study could help plant breeders select an effective mutagenic induction dose rate in wheat.

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Biological Effect of Gamma Rays According to Exposure Time on Germination and Plant Growth in Wheat

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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