Bioluminescence reporter gene-based detection of microRNAs

Hae Young Ko; Young Sik Lee; Soonhag Kim

doi:10.1007/978-1-62703-718-1-7

Bioluminescence reporter gene-based detection of microRNAs

Hae Young Ko, Young Sik Lee, Soonhag Kim

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

MicroRNAs (miRNAs) are an abundant class of small noncoding RNA molecules that inhibit the expression of cognate genes in multicellular organisms. These small RNAs have been demonstrated to play crucial roles in a variety of biological processes including cell differentiation, proliferation, and survival. Knowledge of specific expression patterns of miRNAs is critical for functional studies. Here, we describe a bioluminescence reporter gene-based method to measure miRNA activity in cultured cells and mice using a Gaussia luciferase reporter gene controlled by miRNA binding sites in its 3′untranslated region. This method can be used to noninvasively monitor the expression patterns of functionally active miRNAs involved in different biological processes or diseases in mice.

Original language	English
Title of host publication	Methods in Molecular Biology
Pages	85-95
Number of pages	11
Volume	1098
DOIs	https://doi.org/10.1007/978-1-62703-718-1-7
Publication status	Published - 2014 Jan 10

Publication series

Name	Methods in Molecular Biology
Volume	1098
ISSN (Print)	10643745

Keywords

Bioluminescence reporter gene
Gaussia luciferase
Imaging
MicroRNA

ASJC Scopus subject areas

Molecular Biology
Genetics

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10.1007/978-1-62703-718-1-7

Cite this

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abstract = "MicroRNAs (miRNAs) are an abundant class of small noncoding RNA molecules that inhibit the expression of cognate genes in multicellular organisms. These small RNAs have been demonstrated to play crucial roles in a variety of biological processes including cell differentiation, proliferation, and survival. Knowledge of specific expression patterns of miRNAs is critical for functional studies. Here, we describe a bioluminescence reporter gene-based method to measure miRNA activity in cultured cells and mice using a Gaussia luciferase reporter gene controlled by miRNA binding sites in its 3′untranslated region. This method can be used to noninvasively monitor the expression patterns of functionally active miRNAs involved in different biological processes or diseases in mice.",

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AB - MicroRNAs (miRNAs) are an abundant class of small noncoding RNA molecules that inhibit the expression of cognate genes in multicellular organisms. These small RNAs have been demonstrated to play crucial roles in a variety of biological processes including cell differentiation, proliferation, and survival. Knowledge of specific expression patterns of miRNAs is critical for functional studies. Here, we describe a bioluminescence reporter gene-based method to measure miRNA activity in cultured cells and mice using a Gaussia luciferase reporter gene controlled by miRNA binding sites in its 3′untranslated region. This method can be used to noninvasively monitor the expression patterns of functionally active miRNAs involved in different biological processes or diseases in mice.

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KW - Imaging

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Bioluminescence reporter gene-based detection of microRNAs

Abstract

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Keywords

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