Biomarker gene response in male Medaka (Oryzias latipes) chronically exposed to silver nanoparticle

Chi Hoa Pham, Jongheop Yi, Man Bock Gu

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55 Citations (Scopus)


The chronic toxicity test has been conducted for twenty-eight days to characterize the hepatic expression levels of eight stress-related genes after exposing Medaka to two doses of silver nitrate or a silver nanoparticle (Ag-NP) using real time RT-PCR analysis. This extends our previously published work to include three additional biomarkers and three later time points. In comparing with the control, the significant induction of MT and GST genes in livers of the fish exposed to 1μg/l Ag-NPs was observed at various time points during the test period. The Orla C3-1 (Medaka) gene was slightly induced only with 1μg/l Ag-NPs at 7-day exposure while the suppression of p53 and HSP70 was recorded in all exposures at the end of the test. The gene encoding transferrin was repressed at day 21 by both silver types and at every exposure dosage. These results revealed that the Ag-NPs increase metal detoxification, oxidative and inflammatory stress, and finally stimulate immune responses in Medaka. The conspicuous induction of choriogenin L and vitellogenin 1 in male fish exposed to Ag-NPs, especially at 7- and 21-day, compared with the exposures of AgNO 3 or control was the first attempt to examine estrogenic effects of Ag-NPs.

Original languageEnglish
Pages (from-to)239-245
Number of pages7
JournalEcotoxicology and Environmental Safety
Publication statusPublished - 2012 Apr 1

Bibliographical note

Funding Information:
The authors are grateful to colleagues in Toxicology Research Center, Korea Research Institute of Chemical Technology, (Daejon, Korea) for their kind support in providing the Japanese Medaka stocks. This work has been financially supported by the Eco-technopia 21 project from Korea Ministry of Environment (Project # 091-071-006 ).

Copyright 2012 Elsevier B.V., All rights reserved.


  • Biomarker
  • Chronic toxicity
  • Medaka
  • Nanotoxicity
  • Real-time PCR
  • Silver nanoparticle

ASJC Scopus subject areas

  • Pollution
  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis


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