Biomimetic spinning of silk fibers and: In situ cell encapsulation

Jie Cheng, Doyeun Park, Yesl Jun, Jaeseo Lee, Jinho Hyun, Sang Hoon Lee

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

In situ embedding of sensitive materials (e.g., cells and proteins) in silk fibers without damage presents a significant challenge due to the lack of mild and efficient methods. Here, we report the development of a microfluidic chip-based method for preparation of meter-long silk fibroin (SF) hydrogel fibers by mimicking the silkworm-spinning process. For the spinning of SF fibers, alginate was used as a sericin-like material to induce SF phase separation and entrap liquid SFs, making it possible to shape the outline of SF-based fibers under mild physicochemical conditions. L929 fibroblasts were encapsulated in the fibric hydrogel and displayed excellent viability. Cell-laden SF fibric hydrogels prepared using our method offer a new type of SF-based biomedical device with potential utility in biomedicine.

Original languageEnglish
Pages (from-to)2654-2661
Number of pages8
JournalLab on a Chip
Volume16
Issue number14
DOIs
Publication statusPublished - 2016

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation of Korea (NRF-2015R1A2A1A09004998), Republic of Korea.

Publisher Copyright:
© 2016 The Royal Society of Chemistry.

Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • General Chemistry
  • Biomedical Engineering

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