Biomimetic virus-based colourimetric sensors

Jin Woo Oh; Woo Jae Chung; Kwang Heo; Hyo Eon Jin; Byung Yang Lee; Eddie Wang; Chris Zueger; Winnie Wong; Joel Meyer; Chuntae Kim; So Young Lee; Won Geun Kim; Marcin Zemla; Manfred Auer; Alexander Hexemer; Seung Wuk Lee

doi:10.1038/ncomms4043

Biomimetic virus-based colourimetric sensors

Jin Woo Oh, Woo Jae Chung, Kwang Heo, Hyo Eon Jin, Byung Yang Lee, Eddie Wang, Chris Zueger, Winnie Wong, Joel Meyer, Chuntae Kim, So Young Lee, Won Geun Kim, Marcin Zemla, Manfred Auer, Alexander Hexemer, Seung Wuk Lee

School of Mechanical Engineering

Research output: Contribution to journal › Article › peer-review

190 Citations (Scopus)

Abstract

Many materials in nature change colours in response to stimuli, making them attractive for use as sensor platform. However, both natural materials and their synthetic analogues lack selectivity towards specific chemicals, and introducing such selectivity remains a challenge. Here we report the self-assembly of genetically engineered viruses (M13 phage) into target-specific, colourimetric biosensors. The sensors are composed of phage-bundle nanostructures and exhibit viewing-angle independent colour, similar to collagen structures in turkey skin. On exposure to various volatile organic chemicals, the structures rapidly swell and undergo distinct colour changes. Furthermore, sensors composed of phage displaying trinitrotoluene (TNT)-binding peptide motifs identified from a phage display selectively distinguish TNT down to 300 p.p.b. over similarly structured chemicals. Our tunable, colourimetric sensors can be useful for the detection of a variety of harmful toxicants and pathogens to protect human health and national security.

Original language	English
Article number	3043
Journal	Nature communications
Volume	5
DOIs	https://doi.org/10.1038/ncomms4043
Publication status	Published - 2014 Jan 21

ASJC Scopus subject areas

Chemistry(all)
Biochemistry, Genetics and Molecular Biology(all)
Physics and Astronomy(all)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1038/ncomms4043

Cite this

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title = "Biomimetic virus-based colourimetric sensors",

abstract = "Many materials in nature change colours in response to stimuli, making them attractive for use as sensor platform. However, both natural materials and their synthetic analogues lack selectivity towards specific chemicals, and introducing such selectivity remains a challenge. Here we report the self-assembly of genetically engineered viruses (M13 phage) into target-specific, colourimetric biosensors. The sensors are composed of phage-bundle nanostructures and exhibit viewing-angle independent colour, similar to collagen structures in turkey skin. On exposure to various volatile organic chemicals, the structures rapidly swell and undergo distinct colour changes. Furthermore, sensors composed of phage displaying trinitrotoluene (TNT)-binding peptide motifs identified from a phage display selectively distinguish TNT down to 300 p.p.b. over similarly structured chemicals. Our tunable, colourimetric sensors can be useful for the detection of a variety of harmful toxicants and pathogens to protect human health and national security.",

author = "Oh, {Jin Woo} and Chung, {Woo Jae} and Kwang Heo and Jin, {Hyo Eon} and Lee, {Byung Yang} and Eddie Wang and Chris Zueger and Winnie Wong and Joel Meyer and Chuntae Kim and Lee, {So Young} and Kim, {Won Geun} and Marcin Zemla and Manfred Auer and Alexander Hexemer and Lee, {Seung Wuk}",

note = "Funding Information: This research was performed under the supports of the Center of Integrated Nano-mechanical Systems (COINS) of the National Science Foundation (grant number EEC-0832819). S.-W.L. acknowledges the support from Defense Acquisition Program Administration and Agency for Defense Development under the contract (ADD-10-70-06-02) in South Korea. J.-W.O. acknowledges the support by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2013R1A1A1008276) and the support by the Pioneer Research Center Program through the National Research Foundation of Korea funded by the Ministry of Science, ICT & Future Planning (NRF-2013M3C1A3065522). This work is also supported in part by the Samsung Advanced Institute of Technology (SAIT){\textquoteright}s Global Research Outreach (GRO) Program. We thank Valerie Burtchett for the Turkey images.",

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AU - Oh, Jin Woo

AU - Chung, Woo Jae

AU - Heo, Kwang

AU - Jin, Hyo Eon

AU - Lee, Byung Yang

AU - Wang, Eddie

AU - Zueger, Chris

AU - Wong, Winnie

AU - Meyer, Joel

AU - Kim, Chuntae

AU - Lee, So Young

AU - Kim, Won Geun

AU - Zemla, Marcin

AU - Auer, Manfred

AU - Hexemer, Alexander

AU - Lee, Seung Wuk

N1 - Funding Information: This research was performed under the supports of the Center of Integrated Nano-mechanical Systems (COINS) of the National Science Foundation (grant number EEC-0832819). S.-W.L. acknowledges the support from Defense Acquisition Program Administration and Agency for Defense Development under the contract (ADD-10-70-06-02) in South Korea. J.-W.O. acknowledges the support by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2013R1A1A1008276) and the support by the Pioneer Research Center Program through the National Research Foundation of Korea funded by the Ministry of Science, ICT & Future Planning (NRF-2013M3C1A3065522). This work is also supported in part by the Samsung Advanced Institute of Technology (SAIT)’s Global Research Outreach (GRO) Program. We thank Valerie Burtchett for the Turkey images.

PY - 2014/1/21

Y1 - 2014/1/21

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Biomimetic virus-based colourimetric sensors

Abstract

ASJC Scopus subject areas

UN SDGs

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