Biomolecular Imaging of Regeneration of Zebrafish Caudal Fins Using High Spatial Resolution Ambient Mass Spectrometry

Jae Young Kim, Sun Young Lee, Hyunmin Kim, Ji Won Park, Dong Kwon Lim, Dae Won Moon

    Research output: Contribution to journalArticlepeer-review

    15 Citations (Scopus)

    Abstract

    We observed the molecular distribution changes that occurred during the regeneration of fresh zebrafish caudal fins using the recently developed ambient high-resolution mass spectrometry (MS) imaging technique of atmospheric pressure-nanoparticle and plasma-assisted laser desorption ionization (AP-nanoPALDI). AP-nanoPALDI analyses of fresh zebrafish caudal fins revealed that the small molecules, including neurotransmitters, amino acids, lipids, and metabolites of the regenerated area, were more evenly distributed throughout the bony rays and inter-ray mesenchymal tissues compared to the original area in the early stage. Zebrafish caudal fins of less than 200 μm thickness can be very useful for tissue regeneration studies using ambient MS imaging by providing sufficient biomolecular information at the molecular level for wound-healing studies. AP-nanoPALDI imaging was compared with a complementary MS imaging tool, surface sensitive time-of-flight secondary ion MS (ToF-SIMS).

    Original languageEnglish
    Pages (from-to)12723-12730
    Number of pages8
    JournalAnalytical chemistry
    Volume90
    Issue number21
    DOIs
    Publication statusPublished - 2018 Nov 6

    Bibliographical note

    Publisher Copyright:
    © 2018 American Chemical Society.

    ASJC Scopus subject areas

    • Analytical Chemistry

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