Abstract
Streptomyces venezuelae YJ028, bearing a deletion of the entire biosynthetic gene cluster encoding the pikromycin polyketide synthases and desosamine biosynthetic enzymes, was used as a bioconversion system for combinatorial biosynthesis of glycosylated derivatives of tylosin. Two engineered deoxysugar biosynthetic pathways for the biosynthesis of TDP-3-O-demethyl-D-chalcose or TDP-Lrhamnose in conjunction with the glycosyltransferaseauxiliary protein pair DesVII/DesVIII were expressed in a S. venezuelae YJ028 mutant strain. Supplementation of each mutant strain capable of producing TDP-3-O-demethyl- D-chalcose or TDP-L-rhamnose with tylosin aglycone tylactone resulted in the production of the 3-O-demethyl- D-chalcose, D-quinovose, or L-rhamnose-glycosylated tylactone.
Original language | English |
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Pages (from-to) | 613-616 |
Number of pages | 4 |
Journal | Journal of microbiology and biotechnology |
Volume | 21 |
Issue number | 6 |
DOIs | |
Publication status | Published - 2011 Jun |
Externally published | Yes |
Keywords
- Combinatorial biosynthesis
- Streptomyces venezuelae
- Tylosin
ASJC Scopus subject areas
- Biotechnology
- Applied Microbiology and Biotechnology