Biosynthesis of L-fucose and L-fuculose using engineered Saccharomyces cerevisiae

Jungyeon Kim, Yu Eun Cheong, Sora Yu, Eun Ju Yun, Yong Su Jin, Kyoung Heon Kim

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Recently, L-fucose has received considerable attention as a potential cosmetic agent and substrate for human oligosaccharide synthesis. To date, only two methods have been reported for L-fucose biosynthesis: microbial biosynthesis based on the GDP-L-fucose pathway and enzymatic conversion using the L-fucose metabolic pathway enzymes. The lack of efficient biosynthetic methods has increased the price of L-fucose and other valuable chemicals synthesized from L-fucose. In this study, we introduced the L-fucose metabolic pathway from Escherichia coli into Saccharomyces cerevisiae and reverse-synthesized 7.75 mg/L L-fucose and 1.55 mg/L L-fuculose with lactaldehyde and glucose as substrates. L-fuculose was synthesized as a by-product of L-fucose biosynthesis. Conversion of lactaldehyde to 1,2 propanediol by an unknown oxidoreductase from S. cerevisiae is the bottleneck for fucose synthesis. To the best of our knowledge, this study is the first to reverse synthesize L-fucose and L-fuculose using whole cells via L-fucose metabolism.

Original languageEnglish
Pages (from-to)152-156
Number of pages5
JournalProcess Biochemistry
Volume132
DOIs
Publication statusPublished - 2023 Sept

Bibliographical note

Funding Information:
This work was supported by the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry, and Fisheries and funded by the Ministry of Agriculture, Food, and Rural Affairs ( 32136-05-1-SB010 ) and the Ministry of Trade, Industry and Energy ( 20018132 ). KHK also received grant support from Korea University and the Institute of Biomedical Science and Food Safety at the Korea University Food Safety Hall, Korea University .

Publisher Copyright:
© 2023 Elsevier Ltd

Keywords

  • Biosynthesis
  • L-Fucose
  • Saccharomyces cerevisiae
  • l-Fuculose

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

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