Abstract
The purpose of this study was to evaluate the potential of porous poly (D,L-lactic-co-glycolic acid) (PLGA) microspheres (PMSs) immobilized on biphasic calcium phosphate nanoparticles (BCP NPs) (BCP-IM-PMSs) to enhance osteogenic activity. PMSs were fabricated using a fluidic device, and their surfaces were modified with L-lysine (aminated-PMSs), whereas the BCP NPs were modified with heparin-dopamine (Hep-DOPA) to obtain heparinized-BCP (Hep-BCP) NPs. BCP-IM-PMSs were fabricated via electrostatic interactions between the Hep-BCP NPs and aminated-PMSs. The fabricated BCP-IM-PMSs showed an interconnected pore structure. In vitro studies showed that MG-63 cells cultured on BCP-IM-PMSs had increased alkaline phosphatase activity, calcium content, and mRNA expression of osteocalcin (OCN) and osteopontin (OPN) compared with cells cultured on PMSs. These data suggest that BCP NP-immobilized PMSs have the potential to enhance osteogenic activity.
Original language | English |
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Article number | 297 |
Journal | Polymers |
Volume | 9 |
Issue number | 7 |
DOIs | |
Publication status | Published - 2017 Jul 21 |
Externally published | Yes |
Keywords
- Biphasic calcium phosphate (BCP)
- MG-63 cells
- Osteogenic activity
- Porous microspheres (PMSs)
ASJC Scopus subject areas
- Chemistry(all)
- Polymers and Plastics