Biphasic calcium phosphate (BCP)-immobilized porous poly (D,L-lactic-co-glycolic acid) microspheres enhance osteogenic activities of osteoblasts

Kyu Sik Shim; Sung Eun Kim; Young Pil Yun; Somang Choi; Hak Jun Kim; Kyeongsoon Park; Hae Ryong Song

doi:10.3390/polym9070297

Biphasic calcium phosphate (BCP)-immobilized porous poly (D,L-lactic-co-glycolic acid) microspheres enhance osteogenic activities of osteoblasts

Kyu Sik Shim, Sung Eun Kim, Young Pil Yun, Somang Choi, Hak Jun Kim, Kyeongsoon Park, Hae Ryong Song

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

The purpose of this study was to evaluate the potential of porous poly (D,L-lactic-co-glycolic acid) (PLGA) microspheres (PMSs) immobilized on biphasic calcium phosphate nanoparticles (BCP NPs) (BCP-IM-PMSs) to enhance osteogenic activity. PMSs were fabricated using a fluidic device, and their surfaces were modified with L-lysine (aminated-PMSs), whereas the BCP NPs were modified with heparin-dopamine (Hep-DOPA) to obtain heparinized-BCP (Hep-BCP) NPs. BCP-IM-PMSs were fabricated via electrostatic interactions between the Hep-BCP NPs and aminated-PMSs. The fabricated BCP-IM-PMSs showed an interconnected pore structure. In vitro studies showed that MG-63 cells cultured on BCP-IM-PMSs had increased alkaline phosphatase activity, calcium content, and mRNA expression of osteocalcin (OCN) and osteopontin (OPN) compared with cells cultured on PMSs. These data suggest that BCP NP-immobilized PMSs have the potential to enhance osteogenic activity.

Original language	English
Article number	297
Journal	Polymers
Volume	9
Issue number	7
DOIs	https://doi.org/10.3390/polym9070297
Publication status	Published - 2017 Jul 21
Externally published	Yes

Keywords

Biphasic calcium phosphate (BCP)
MG-63 cells
Osteogenic activity
Porous microspheres (PMSs)

ASJC Scopus subject areas

Chemistry(all)
Polymers and Plastics

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title = "Biphasic calcium phosphate (BCP)-immobilized porous poly (D,L-lactic-co-glycolic acid) microspheres enhance osteogenic activities of osteoblasts",

abstract = "The purpose of this study was to evaluate the potential of porous poly (D,L-lactic-co-glycolic acid) (PLGA) microspheres (PMSs) immobilized on biphasic calcium phosphate nanoparticles (BCP NPs) (BCP-IM-PMSs) to enhance osteogenic activity. PMSs were fabricated using a fluidic device, and their surfaces were modified with L-lysine (aminated-PMSs), whereas the BCP NPs were modified with heparin-dopamine (Hep-DOPA) to obtain heparinized-BCP (Hep-BCP) NPs. BCP-IM-PMSs were fabricated via electrostatic interactions between the Hep-BCP NPs and aminated-PMSs. The fabricated BCP-IM-PMSs showed an interconnected pore structure. In vitro studies showed that MG-63 cells cultured on BCP-IM-PMSs had increased alkaline phosphatase activity, calcium content, and mRNA expression of osteocalcin (OCN) and osteopontin (OPN) compared with cells cultured on PMSs. These data suggest that BCP NP-immobilized PMSs have the potential to enhance osteogenic activity.",

keywords = "Biphasic calcium phosphate (BCP), MG-63 cells, Osteogenic activity, Porous microspheres (PMSs)",

author = "Shim, {Kyu Sik} and Kim, {Sung Eun} and Yun, {Young Pil} and Somang Choi and Kim, {Hak Jun} and Kyeongsoon Park and Song, {Hae Ryong}",

note = "Publisher Copyright: {\textcopyright} 2017 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2017",

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doi = "10.3390/polym9070297",

language = "English",

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T1 - Biphasic calcium phosphate (BCP)-immobilized porous poly (D,L-lactic-co-glycolic acid) microspheres enhance osteogenic activities of osteoblasts

AU - Shim, Kyu Sik

AU - Kim, Sung Eun

AU - Yun, Young Pil

AU - Choi, Somang

AU - Kim, Hak Jun

AU - Park, Kyeongsoon

AU - Song, Hae Ryong

PY - 2017/7/21

Y1 - 2017/7/21

N2 - The purpose of this study was to evaluate the potential of porous poly (D,L-lactic-co-glycolic acid) (PLGA) microspheres (PMSs) immobilized on biphasic calcium phosphate nanoparticles (BCP NPs) (BCP-IM-PMSs) to enhance osteogenic activity. PMSs were fabricated using a fluidic device, and their surfaces were modified with L-lysine (aminated-PMSs), whereas the BCP NPs were modified with heparin-dopamine (Hep-DOPA) to obtain heparinized-BCP (Hep-BCP) NPs. BCP-IM-PMSs were fabricated via electrostatic interactions between the Hep-BCP NPs and aminated-PMSs. The fabricated BCP-IM-PMSs showed an interconnected pore structure. In vitro studies showed that MG-63 cells cultured on BCP-IM-PMSs had increased alkaline phosphatase activity, calcium content, and mRNA expression of osteocalcin (OCN) and osteopontin (OPN) compared with cells cultured on PMSs. These data suggest that BCP NP-immobilized PMSs have the potential to enhance osteogenic activity.

AB - The purpose of this study was to evaluate the potential of porous poly (D,L-lactic-co-glycolic acid) (PLGA) microspheres (PMSs) immobilized on biphasic calcium phosphate nanoparticles (BCP NPs) (BCP-IM-PMSs) to enhance osteogenic activity. PMSs were fabricated using a fluidic device, and their surfaces were modified with L-lysine (aminated-PMSs), whereas the BCP NPs were modified with heparin-dopamine (Hep-DOPA) to obtain heparinized-BCP (Hep-BCP) NPs. BCP-IM-PMSs were fabricated via electrostatic interactions between the Hep-BCP NPs and aminated-PMSs. The fabricated BCP-IM-PMSs showed an interconnected pore structure. In vitro studies showed that MG-63 cells cultured on BCP-IM-PMSs had increased alkaline phosphatase activity, calcium content, and mRNA expression of osteocalcin (OCN) and osteopontin (OPN) compared with cells cultured on PMSs. These data suggest that BCP NP-immobilized PMSs have the potential to enhance osteogenic activity.

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KW - Porous microspheres (PMSs)

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Biphasic calcium phosphate (BCP)-immobilized porous poly (D,L-lactic-co-glycolic acid) microspheres enhance osteogenic activities of osteoblasts

Abstract

Keywords

ASJC Scopus subject areas

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