Blue-Red LED wavelength shifting strategy for enhancing beta-carotene production from halotolerant microalga, Dunaliella salina

Sang Il Han; Sok Kim; Changsu Lee; Yoon E. Choi

doi:10.1007/s12275-019-8420-4

Blue-Red LED wavelength shifting strategy for enhancing beta-carotene production from halotolerant microalga, Dunaliella salina

Sang Il Han, Sok Kim, Changsu Lee, Yoon E. Choi

Division of Environmental Science and Ecological Engineering

Research output: Contribution to journal › Article › peer-review

42 Citations (Scopus)

Abstract

In the present study, to improve the photosynthetic betacarotene productivity of Dunaliella salina, a blue-red LED wavelength-shifting system (B-R system) was investigated. Dunaliella salina under the B-R system showed enhanced density and beta-carotene productivity compared to D. salina cultivated under single light-emitting diode light wavelengths (blue, white, and red light-emitting diode). Additionally, we developed blue light-adapted D. salina (ALE-D. salina) using an adaptive laboratory evolution (ALE) approach. In combination with the B-R system applied to ALE-D. salina (ALE B-R system), the beta-carotene concentration (33.94 ± 0.52 μM) was enhanced by 19.7% compared to that observed for the non-ALE-treated wild-type of D. salina (intact D. salina) under the B-R system (28.34 ± 0.24 μM).

Original language	English
Pages (from-to)	101-106
Number of pages	6
Journal	Journal of Microbiology
Volume	57
Issue number	2
DOIs	https://doi.org/10.1007/s12275-019-8420-4
Publication status	Published - 2019 Feb 1

Keywords

Dunaliella salina
adaptive laboratory evolution
beta-carotene
light-emitting diode
wavelength shift

ASJC Scopus subject areas

Microbiology
Applied Microbiology and Biotechnology

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10.1007/s12275-019-8420-4

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title = "Blue-Red LED wavelength shifting strategy for enhancing beta-carotene production from halotolerant microalga, Dunaliella salina",

abstract = "In the present study, to improve the photosynthetic betacarotene productivity of Dunaliella salina, a blue-red LED wavelength-shifting system (B-R system) was investigated. Dunaliella salina under the B-R system showed enhanced density and beta-carotene productivity compared to D. salina cultivated under single light-emitting diode light wavelengths (blue, white, and red light-emitting diode). Additionally, we developed blue light-adapted D. salina (ALE-D. salina) using an adaptive laboratory evolution (ALE) approach. In combination with the B-R system applied to ALE-D. salina (ALE B-R system), the beta-carotene concentration (33.94 ± 0.52 μM) was enhanced by 19.7% compared to that observed for the non-ALE-treated wild-type of D. salina (intact D. salina) under the B-R system (28.34 ± 0.24 μM).",

keywords = "Dunaliella salina, adaptive laboratory evolution, beta-carotene, light-emitting diode, wavelength shift",

author = "Han, {Sang Il} and Sok Kim and Changsu Lee and Choi, {Yoon E.}",

note = "Funding Information: This work was supported by the Government of South Korea through the National Research Foundation of Korea (NRF-2016R1D1A1B03932773). This research was also supported by the Marine Biotechnology Program of the Korea Institute of Marine Science and Technology Promotion (KIMST) funded by the Ministry of Oceans and Fisheries (MOF) (No. 20170488). Publisher Copyright: {\textcopyright} 2019, The Microbiological Society of Korea and Springer Nature B.V.",

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N1 - Funding Information: This work was supported by the Government of South Korea through the National Research Foundation of Korea (NRF-2016R1D1A1B03932773). This research was also supported by the Marine Biotechnology Program of the Korea Institute of Marine Science and Technology Promotion (KIMST) funded by the Ministry of Oceans and Fisheries (MOF) (No. 20170488). Publisher Copyright: © 2019, The Microbiological Society of Korea and Springer Nature B.V.

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N2 - In the present study, to improve the photosynthetic betacarotene productivity of Dunaliella salina, a blue-red LED wavelength-shifting system (B-R system) was investigated. Dunaliella salina under the B-R system showed enhanced density and beta-carotene productivity compared to D. salina cultivated under single light-emitting diode light wavelengths (blue, white, and red light-emitting diode). Additionally, we developed blue light-adapted D. salina (ALE-D. salina) using an adaptive laboratory evolution (ALE) approach. In combination with the B-R system applied to ALE-D. salina (ALE B-R system), the beta-carotene concentration (33.94 ± 0.52 μM) was enhanced by 19.7% compared to that observed for the non-ALE-treated wild-type of D. salina (intact D. salina) under the B-R system (28.34 ± 0.24 μM).

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Blue-Red LED wavelength shifting strategy for enhancing beta-carotene production from halotolerant microalga, Dunaliella salina

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