BODIPY/Nile-Red-Based Efficient FRET Pair: Selective Assay of Endoplasmic Reticulum Membrane Fluidity

Zhigang Yang; Youngjin Wi; Young Min Yoon; Peter Verwilst; Joo Hee Jang; Tae Woo Kim; Chulhun Kang; Jong Seung Kim

doi:10.1002/asia.201501060

BODIPY/Nile-Red-Based Efficient FRET Pair: Selective Assay of Endoplasmic Reticulum Membrane Fluidity

Zhigang Yang, Youngjin Wi, Young Min Yoon, Peter Verwilst, Joo Hee Jang, Tae Woo Kim, Chulhun Kang, Jong Seung Kim

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

We synthesized a boron-dipyrromethene (BODIPY)/Nile Red hybrid probe capable of selectively recognizing fluidity changes in the endoplasmic reticulum (ER) membrane due to its preferential localization to the ER and strong energy transfer from BODIPY to the Nile Red moiety, emitting only in nonaqueous environments. ER membrane fluidity in HepG2 cells was markedly reduced by a cell model of metabolic syndrome.

Original language	English
Pages (from-to)	527-531
Number of pages	5
Journal	Chemistry - An Asian Journal
Volume	11
Issue number	4
DOIs	https://doi.org/10.1002/asia.201501060
Publication status	Published - 2016 Feb 18

Bibliographical note

Funding Information:
This work was supported by NRF (No. 2014R1A2A1A11052325, CK), (No. 2013R1A1A2065643, TWK), and CRI (No. 2009- 0081566, JSK) project grants from the National Research Foundation of Korea. PV is supported by a Korea University grant.

Publisher Copyright:
© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Keywords

FRET
endoplasmic reticulum
fluorescence
membrane fluidity
metabolic syndrome

ASJC Scopus subject areas

Biochemistry
Organic Chemistry

Access to Document

10.1002/asia.201501060

Cite this

@article{cf7447719bc34710b4ead641c8eff470,

title = "BODIPY/Nile-Red-Based Efficient FRET Pair: Selective Assay of Endoplasmic Reticulum Membrane Fluidity",

abstract = "We synthesized a boron-dipyrromethene (BODIPY)/Nile Red hybrid probe capable of selectively recognizing fluidity changes in the endoplasmic reticulum (ER) membrane due to its preferential localization to the ER and strong energy transfer from BODIPY to the Nile Red moiety, emitting only in nonaqueous environments. ER membrane fluidity in HepG2 cells was markedly reduced by a cell model of metabolic syndrome.",

keywords = "FRET, endoplasmic reticulum, fluorescence, membrane fluidity, metabolic syndrome",

author = "Zhigang Yang and Youngjin Wi and Yoon, {Young Min} and Peter Verwilst and Jang, {Joo Hee} and Kim, {Tae Woo} and Chulhun Kang and Kim, {Jong Seung}",

note = "Funding Information: This work was supported by NRF (No. 2014R1A2A1A11052325, CK), (No. 2013R1A1A2065643, TWK), and CRI (No. 2009- 0081566, JSK) project grants from the National Research Foundation of Korea. PV is supported by a Korea University grant. Publisher Copyright: {\textcopyright} 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.",

year = "2016",

month = feb,

day = "18",

doi = "10.1002/asia.201501060",

language = "English",

volume = "11",

pages = "527--531",

journal = "Chemistry - An Asian Journal",

issn = "1861-4728",

publisher = "John Wiley and Sons Ltd",

number = "4",

}

TY - JOUR

T1 - BODIPY/Nile-Red-Based Efficient FRET Pair

T2 - Selective Assay of Endoplasmic Reticulum Membrane Fluidity

AU - Yang, Zhigang

AU - Wi, Youngjin

AU - Yoon, Young Min

AU - Verwilst, Peter

AU - Jang, Joo Hee

AU - Kim, Tae Woo

AU - Kang, Chulhun

AU - Kim, Jong Seung

N1 - Funding Information: This work was supported by NRF (No. 2014R1A2A1A11052325, CK), (No. 2013R1A1A2065643, TWK), and CRI (No. 2009- 0081566, JSK) project grants from the National Research Foundation of Korea. PV is supported by a Korea University grant. Publisher Copyright: © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

PY - 2016/2/18

Y1 - 2016/2/18

N2 - We synthesized a boron-dipyrromethene (BODIPY)/Nile Red hybrid probe capable of selectively recognizing fluidity changes in the endoplasmic reticulum (ER) membrane due to its preferential localization to the ER and strong energy transfer from BODIPY to the Nile Red moiety, emitting only in nonaqueous environments. ER membrane fluidity in HepG2 cells was markedly reduced by a cell model of metabolic syndrome.

AB - We synthesized a boron-dipyrromethene (BODIPY)/Nile Red hybrid probe capable of selectively recognizing fluidity changes in the endoplasmic reticulum (ER) membrane due to its preferential localization to the ER and strong energy transfer from BODIPY to the Nile Red moiety, emitting only in nonaqueous environments. ER membrane fluidity in HepG2 cells was markedly reduced by a cell model of metabolic syndrome.

KW - FRET

KW - endoplasmic reticulum

KW - fluorescence

KW - membrane fluidity

KW - metabolic syndrome

UR - http://www.scopus.com/inward/record.url?scp=84959561629&partnerID=8YFLogxK

U2 - 10.1002/asia.201501060

DO - 10.1002/asia.201501060

M3 - Article

C2 - 26537552

AN - SCOPUS:84959561629

SN - 1861-4728

VL - 11

SP - 527

EP - 531

JO - Chemistry - An Asian Journal

JF - Chemistry - An Asian Journal

IS - 4

ER -

BODIPY/Nile-Red-Based Efficient FRET Pair: Selective Assay of Endoplasmic Reticulum Membrane Fluidity

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this