TY - JOUR
T1 - Bone marrow-derived cells or C-X-C motif chemokine 12 (CXCL12) treatment improve thin endometrium in a mouse model
AU - Yi, Kyong Wook
AU - Mamillapalli, Ramanaiah
AU - Sahin, Cagdas
AU - Song, Jaeyen
AU - Tal, Reshef
AU - Taylor, Hugh S.
N1 - Funding Information:
1Department of Obstetrics and Gynecology, Reproductive Sciences, Yale School of Medicine, New Haven, Connecticut, USA and 2Department of Obstetrics and Gynecology, Korea University College of Medicine, Seoul, Republic of Korea ∗Correspondence: Department of Obstetrics, Gynecology and Reproductive Sciences, Yale School of Medicine, 310 Cedar Street, New Haven, CT 06510, USA. Tel: +203-737-8082; Fax: +203-785-4713; E-mail: ramana.mamillapalli@yale.edu †Grant Support: This work was supported by the National Institutes of Health NIH RO1 HD052668 to HST Conference Presentation: Presented in part at the 65th Annual Meeting of the Society for Reproductive Investigation (SRI), 6–10 March 2018, San Diego, CA, USA (Abstract O-121). Edited by Dr. Haibin Wang, PhD, Xiamen University
Funding Information:
This work was supported by the National Institutes of Health NIH RO1 HD052668 to HST
Publisher Copyright:
© The Author(s) 2018. Published by Oxford University Press on behalf of Society for the Study of Reproduction. All rights reserved.
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Successful implantation and pregnancy is dependent on sufficient endometrial growth during each reproductive cycle. Here, we report the therapeutic effect of either bone marrow-derived cells (BMDCs) or the stem cell chemo-attractant C-X-C motif chemokine 12 (CXCL12) on endometrial receptivity in a murine ethanol induced thin endometrium model. Endometrial epithelial area was significantly increased in mice treated with BMDCs, CXCL12, or by co-treatment with both compared with PBS-treated controls. Ki-67 and CD31 immunoreactivity was significantly higher in mice treated with either BMDCs, CXCL12, or both. The mRNA expression levels of endometrial receptivity markers leukemia inhibitory factor, interleukin-1β, and integrin beta-3 were increased in mice treated with either BMDCs, CXCL12, or both. The mRNA levels of matrix metalloproteinase-2 and -9 were significantly decreased by BMDCs but not by CXCL12. Pregnancy rates and litter size were increased after either treatment. Both BMDCs and CXCL12 displayed a comparable efficacy on endometrial regeneration in mice with thin endometrium. Our findings indicate the potential therapeutic effects of BMDCs and CXCL12 on infertility related to thin endometrium. Bone marrow-derived cells and CXCL12 displayed a comparable efficacy on endometrial regeneration in mice with thin endometrium.
AB - Successful implantation and pregnancy is dependent on sufficient endometrial growth during each reproductive cycle. Here, we report the therapeutic effect of either bone marrow-derived cells (BMDCs) or the stem cell chemo-attractant C-X-C motif chemokine 12 (CXCL12) on endometrial receptivity in a murine ethanol induced thin endometrium model. Endometrial epithelial area was significantly increased in mice treated with BMDCs, CXCL12, or by co-treatment with both compared with PBS-treated controls. Ki-67 and CD31 immunoreactivity was significantly higher in mice treated with either BMDCs, CXCL12, or both. The mRNA expression levels of endometrial receptivity markers leukemia inhibitory factor, interleukin-1β, and integrin beta-3 were increased in mice treated with either BMDCs, CXCL12, or both. The mRNA levels of matrix metalloproteinase-2 and -9 were significantly decreased by BMDCs but not by CXCL12. Pregnancy rates and litter size were increased after either treatment. Both BMDCs and CXCL12 displayed a comparable efficacy on endometrial regeneration in mice with thin endometrium. Our findings indicate the potential therapeutic effects of BMDCs and CXCL12 on infertility related to thin endometrium. Bone marrow-derived cells and CXCL12 displayed a comparable efficacy on endometrial regeneration in mice with thin endometrium.
KW - Bone marrow-derived cells (BMDCs)
KW - CXCL12
KW - Infertility
KW - Thin endometrium
UR - http://www.scopus.com/inward/record.url?scp=85059527940&partnerID=8YFLogxK
U2 - 10.1093/biolre/ioy175
DO - 10.1093/biolre/ioy175
M3 - Article
C2 - 30084961
AN - SCOPUS:85059527940
SN - 0006-3363
VL - 100
SP - 61
EP - 70
JO - Biology of Reproduction
JF - Biology of Reproduction
IS - 1
ER -