Bottom-Up Engineering of Well-Defined 3D Microtissues Using Microplatforms and Biomedical Applications

Geon Hui Lee, Jae Seo Lee, Xiaohong Wang, Sang Hoon Lee

Research output: Contribution to journalReview articlepeer-review

58 Citations (Scopus)


During the last decades, the engineering of well-defined 3D tissues has attracted great attention because it provides in vivo mimicking environment and can be a building block for the engineering of bioartificial organs. In this Review, diverse engineering methods of 3D tissues using microscale devices are introduced. Recent progress of microtechnologies has enabled the development of microplatforms for bottom-up assembly of diverse shaped 3D tissues consisting of various cells. Micro hanging-drop plates, microfluidic chips, and arrayed microwells are the typical examples. The encapsulation of cells in hydrogel microspheres and microfibers allows the engineering of 3D microtissues with diverse shapes. Applications of 3D microtissues in biomedical fields are described, and the future direction of microplatform-based engineering of 3D micro-tissues is discussed.

Original languageEnglish
Pages (from-to)56-74
Number of pages19
JournalAdvanced Healthcare Materials
Issue number1
Publication statusPublished - 2016 Jan 1

Bibliographical note

Publisher Copyright:
© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.


  • 3D cultures
  • Microtissue
  • Regenerative medicine
  • Self-assembly
  • Tissue engineering

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Pharmaceutical Science


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