Abstract
Activation of protein kinase C (PKC) by bryostatin-1 affects various functions of the central nervous system. We explored whether bryostatin-1 influenced synaptic plasticity via a process involving PKC. Our purpose was to examine whether bryostatin-1 affected the induction of hippocampal long-term potentiation (LTP) in Schaffer-collateral fibers (CA1 fibers) of the hippocampus, and/or influenced the intracellular Ca2+ level of hippocampal neurons. We also determined the PKC isoforms involved in these processes. We found that bryostatin-1 strongly facilitated LTP induction, in a dose-dependent manner, upon single-theta burst stimulation (TBS). Further, intracellular Ca2+ levels also increased with increasing concentration of bryostatin-1. The facilitative effects of bryostatin-1 in terms of LTP induction and enhancement of intracellular Ca2+ levels were blocked by specific inhibitors of PKCα and PKCε, but not of PKCδ. Our results suggest that bryostatin-1 is involved in neuronal functioning and facilitates induction of LTP via activation of PKCα and/or PKCε.
Original language | English |
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Pages (from-to) | 348-355 |
Number of pages | 8 |
Journal | Neuroscience |
Volume | 226 |
DOIs | |
Publication status | Published - 2012 Dec 13 |
Bibliographical note
Funding Information:This study was supported by the Mid-career Researcher program through the NRF grant funded by the MEST ( 2011-0027545 ).
Keywords
- Bryostatin-1
- Hippocampus
- Long-term potentiation (LTP)
- Protein kinase C (PKC)
- Synaptic plasticity
ASJC Scopus subject areas
- General Neuroscience