BSA as additive: A simple strategy for practical applications of PNA in bioanalysis

Jieon Lee; Il Soo Park; Henna Kim; Jae Sung Woo; Byong Seok Choi; Dal Hee Min

doi:10.1016/j.bios.2015.02.030

BSA as additive: A simple strategy for practical applications of PNA in bioanalysis

Jieon Lee, Il Soo Park, Henna Kim, Jae Sung Woo, Byong Seok Choi, Dal Hee Min

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

Application of peptide nucleic acid (PNA) in bioanalysis has been limited due to its nonspecific adsorption onto hydrophobic surface in spite of favorable properties such as higher chemical/biological stability, specificity and binding affinity towards target nucleic acids compared to natural nucleic acid probes. Herein, we employed BSA in PNA application to enhance the stability of PNA in hydrophobic containers and improve the sensing performance of the DNA sensor based on graphene oxide (GO) and PNA. Addition of 0.01% BSA in a PNA solution effectively prevented the adsorption of PNA on hydrophobic surface and increased the portion of the effective PNA strands for target binding without interfering duplex formation with a complementary target sequence. In the GO based biosensor using PNA, BSA interrupted the unfavorable adsorption of PNA/DNA duplex on GO surface, while allowing the adsorption of ssPNA, resulting in improvement of the performance of the DNA sensor system by reducing the detection limit by 90-folds.

Original language	English
Pages (from-to)	167-173
Number of pages	7
Journal	Biosensors and Bioelectronics
Volume	69
DOIs	https://doi.org/10.1016/j.bios.2015.02.030
Publication status	Published - 2015 Jul 5
Externally published	Yes

Bibliographical note

Funding Information:
This work was supported by the Basic Science Research Program (2011-0017356, 2011-0020322), International S&T Cooperation Program (2014K1B1A1073716) and the Research Center Program (IBS-R) of IBS (Institute for Basic Science) through the National Research Foundation of Korea (NRF) and Research Program (C0193918) through the SMBA funded by the Korean government ( Ministry of Education, Science and Technology ).

Publisher Copyright:
© 2015 Elsevier B.V.

Keywords

Bioanalysis
Bovine serum albumin
Graphene oxide
Peptide nucleic acid
Signal enhancement

ASJC Scopus subject areas

Biotechnology
Biophysics
Biomedical Engineering
Electrochemistry

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10.1016/j.bios.2015.02.030

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title = "BSA as additive: A simple strategy for practical applications of PNA in bioanalysis",

abstract = "Application of peptide nucleic acid (PNA) in bioanalysis has been limited due to its nonspecific adsorption onto hydrophobic surface in spite of favorable properties such as higher chemical/biological stability, specificity and binding affinity towards target nucleic acids compared to natural nucleic acid probes. Herein, we employed BSA in PNA application to enhance the stability of PNA in hydrophobic containers and improve the sensing performance of the DNA sensor based on graphene oxide (GO) and PNA. Addition of 0.01% BSA in a PNA solution effectively prevented the adsorption of PNA on hydrophobic surface and increased the portion of the effective PNA strands for target binding without interfering duplex formation with a complementary target sequence. In the GO based biosensor using PNA, BSA interrupted the unfavorable adsorption of PNA/DNA duplex on GO surface, while allowing the adsorption of ssPNA, resulting in improvement of the performance of the DNA sensor system by reducing the detection limit by 90-folds.",

keywords = "Bioanalysis, Bovine serum albumin, Graphene oxide, Peptide nucleic acid, Signal enhancement",

author = "Jieon Lee and Park, {Il Soo} and Henna Kim and Woo, {Jae Sung} and Choi, {Byong Seok} and Min, {Dal Hee}",

note = "Funding Information: This work was supported by the Basic Science Research Program (2011-0017356, 2011-0020322), International S&T Cooperation Program (2014K1B1A1073716) and the Research Center Program (IBS-R) of IBS (Institute for Basic Science) through the National Research Foundation of Korea (NRF) and Research Program (C0193918) through the SMBA funded by the Korean government ( Ministry of Education, Science and Technology ). Publisher Copyright: {\textcopyright} 2015 Elsevier B.V.",

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AU - Choi, Byong Seok

AU - Min, Dal Hee

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Y1 - 2015/7/5

N2 - Application of peptide nucleic acid (PNA) in bioanalysis has been limited due to its nonspecific adsorption onto hydrophobic surface in spite of favorable properties such as higher chemical/biological stability, specificity and binding affinity towards target nucleic acids compared to natural nucleic acid probes. Herein, we employed BSA in PNA application to enhance the stability of PNA in hydrophobic containers and improve the sensing performance of the DNA sensor based on graphene oxide (GO) and PNA. Addition of 0.01% BSA in a PNA solution effectively prevented the adsorption of PNA on hydrophobic surface and increased the portion of the effective PNA strands for target binding without interfering duplex formation with a complementary target sequence. In the GO based biosensor using PNA, BSA interrupted the unfavorable adsorption of PNA/DNA duplex on GO surface, while allowing the adsorption of ssPNA, resulting in improvement of the performance of the DNA sensor system by reducing the detection limit by 90-folds.

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BSA as additive: A simple strategy for practical applications of PNA in bioanalysis

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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