Butein effects in colitis and interleukin-6/signal transducer and activator of transcription 3 expression

Sehe Dong Lee, Jung Wan Choe, Beom Jae Lee, Myoung Hee Kang, Moon Kyung Joo, Ji Hoon Kim, Jong Eun Yeon, Jong Jae Park, Jae Seon Kim, Kwan Soo Byun, Young Tae Bak

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)


AIM: To evaluate the effects of butein on inflammatory cytokines, matrix metalloproteinase-9 (MMP-9), and colitis in interleukin (IL)-10-/- mice. METHODS: To synchronize colitis, 8-to 10-wk-old IL-10-/- mice were fed pellet-chow containing piroxicam for 2 wk. Subsequently, phosphate-buffered saline or butein (1 mg/kg per day, ip) was injected for 4 wk. Histologic scores, inflammatory cytokines, MMP-9 and phosphorylated signal transducer and activator of transcription 3 (pSTAT3) expressions were analyzed in IL-10-/- mice and in Colo 205 cells. RESULTS: Butein reduced the colonic inflammatory score by > 50%. Expression levels of IL-6, IL-1β, interferon (IFN)-γ and MMP-9 were decreased in the colons of mice exposed to butein, whereas other inflammatory cytokines (IL-17A, IL-21 and IL-22) were unchanged. Immunohistochemical staining for pSTAT3 and MMP-9 was significantly decreased in the buteintreated groups compared with the controls. Butein inhibited IL-6-induced activation of STAT3 in Colo 205 cells. CONCLUSION: Butein ameliorated colitis in IL-10-/- mice by regulating IL-6/STAT3 and MMP-9 activation.

Original languageEnglish
Pages (from-to)465-474
Number of pages10
JournalWorld journal of gastroenterology
Issue number2
Publication statusPublished - 2015 Jan 14
Externally publishedYes

Bibliographical note

Publisher Copyright:
© The Author(s) 2015. Published by Baishideng Publishing Group Inc. All rights reserved.


  • Butein
  • Colitis
  • Inflammatory bowel disease
  • Interleukin-6/signal transducer and activator of transcription 3
  • Matrix metalloproteinase-9

ASJC Scopus subject areas

  • Gastroenterology


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