Butein effects in colitis and interleukin-6/signal transducer and activator of transcription 3 expression

Sehe Dong Lee; Jung Wan Choe; Beom Jae Lee; Myoung Hee Kang; Moon Kyung Joo; Ji Hoon Kim; Jong Eun Yeon; Jong Jae Park; Jae Seon Kim; Kwan Soo Byun; Young Tae Bak

doi:10.3748/wjg.v21.i2.465

Butein effects in colitis and interleukin-6/signal transducer and activator of transcription 3 expression

Sehe Dong Lee, Jung Wan Choe, Beom Jae Lee, Myoung Hee Kang, Moon Kyung Joo, Ji Hoon Kim, Jong Eun Yeon, Jong Jae Park, Jae Seon Kim, Kwan Soo Byun, Young Tae Bak

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

AIM: To evaluate the effects of butein on inflammatory cytokines, matrix metalloproteinase-9 (MMP-9), and colitis in interleukin (IL)-10^-/- mice. METHODS: To synchronize colitis, 8-to 10-wk-old IL-10^-/- mice were fed pellet-chow containing piroxicam for 2 wk. Subsequently, phosphate-buffered saline or butein (1 mg/kg per day, ip) was injected for 4 wk. Histologic scores, inflammatory cytokines, MMP-9 and phosphorylated signal transducer and activator of transcription 3 (pSTAT3) expressions were analyzed in IL-10^-/- mice and in Colo 205 cells. RESULTS: Butein reduced the colonic inflammatory score by > 50%. Expression levels of IL-6, IL-1β, interferon (IFN)-γ and MMP-9 were decreased in the colons of mice exposed to butein, whereas other inflammatory cytokines (IL-17A, IL-21 and IL-22) were unchanged. Immunohistochemical staining for pSTAT3 and MMP-9 was significantly decreased in the buteintreated groups compared with the controls. Butein inhibited IL-6-induced activation of STAT3 in Colo 205 cells. CONCLUSION: Butein ameliorated colitis in IL-10^-/- mice by regulating IL-6/STAT3 and MMP-9 activation.

Original language	English
Pages (from-to)	465-474
Number of pages	10
Journal	World journal of gastroenterology
Volume	21
Issue number	2
DOIs	https://doi.org/10.3748/wjg.v21.i2.465
Publication status	Published - 2015 Jan 14
Externally published	Yes

Bibliographical note

Publisher Copyright:
© The Author(s) 2015. Published by Baishideng Publishing Group Inc. All rights reserved.

Keywords

Butein
Colitis
Inflammatory bowel disease
Interleukin-6/signal transducer and activator of transcription 3
Matrix metalloproteinase-9

ASJC Scopus subject areas

Gastroenterology

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10.3748/wjg.v21.i2.465

Cite this

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title = "Butein effects in colitis and interleukin-6/signal transducer and activator of transcription 3 expression",

abstract = "AIM: To evaluate the effects of butein on inflammatory cytokines, matrix metalloproteinase-9 (MMP-9), and colitis in interleukin (IL)-10-/- mice. METHODS: To synchronize colitis, 8-to 10-wk-old IL-10-/- mice were fed pellet-chow containing piroxicam for 2 wk. Subsequently, phosphate-buffered saline or butein (1 mg/kg per day, ip) was injected for 4 wk. Histologic scores, inflammatory cytokines, MMP-9 and phosphorylated signal transducer and activator of transcription 3 (pSTAT3) expressions were analyzed in IL-10-/- mice and in Colo 205 cells. RESULTS: Butein reduced the colonic inflammatory score by > 50%. Expression levels of IL-6, IL-1β, interferon (IFN)-γ and MMP-9 were decreased in the colons of mice exposed to butein, whereas other inflammatory cytokines (IL-17A, IL-21 and IL-22) were unchanged. Immunohistochemical staining for pSTAT3 and MMP-9 was significantly decreased in the buteintreated groups compared with the controls. Butein inhibited IL-6-induced activation of STAT3 in Colo 205 cells. CONCLUSION: Butein ameliorated colitis in IL-10-/- mice by regulating IL-6/STAT3 and MMP-9 activation.",

keywords = "Butein, Colitis, Inflammatory bowel disease, Interleukin-6/signal transducer and activator of transcription 3, Matrix metalloproteinase-9",

author = "Lee, {Sehe Dong} and Choe, {Jung Wan} and Lee, {Beom Jae} and Kang, {Myoung Hee} and Joo, {Moon Kyung} and Kim, {Ji Hoon} and Yeon, {Jong Eun} and Park, {Jong Jae} and Kim, {Jae Seon} and Byun, {Kwan Soo} and Bak, {Young Tae}",

year = "2015",

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doi = "10.3748/wjg.v21.i2.465",

language = "English",

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T1 - Butein effects in colitis and interleukin-6/signal transducer and activator of transcription 3 expression

AU - Lee, Sehe Dong

AU - Choe, Jung Wan

AU - Lee, Beom Jae

AU - Kang, Myoung Hee

AU - Joo, Moon Kyung

AU - Kim, Ji Hoon

AU - Yeon, Jong Eun

AU - Park, Jong Jae

AU - Kim, Jae Seon

AU - Byun, Kwan Soo

AU - Bak, Young Tae

PY - 2015/1/14

Y1 - 2015/1/14

N2 - AIM: To evaluate the effects of butein on inflammatory cytokines, matrix metalloproteinase-9 (MMP-9), and colitis in interleukin (IL)-10-/- mice. METHODS: To synchronize colitis, 8-to 10-wk-old IL-10-/- mice were fed pellet-chow containing piroxicam for 2 wk. Subsequently, phosphate-buffered saline or butein (1 mg/kg per day, ip) was injected for 4 wk. Histologic scores, inflammatory cytokines, MMP-9 and phosphorylated signal transducer and activator of transcription 3 (pSTAT3) expressions were analyzed in IL-10-/- mice and in Colo 205 cells. RESULTS: Butein reduced the colonic inflammatory score by > 50%. Expression levels of IL-6, IL-1β, interferon (IFN)-γ and MMP-9 were decreased in the colons of mice exposed to butein, whereas other inflammatory cytokines (IL-17A, IL-21 and IL-22) were unchanged. Immunohistochemical staining for pSTAT3 and MMP-9 was significantly decreased in the buteintreated groups compared with the controls. Butein inhibited IL-6-induced activation of STAT3 in Colo 205 cells. CONCLUSION: Butein ameliorated colitis in IL-10-/- mice by regulating IL-6/STAT3 and MMP-9 activation.

AB - AIM: To evaluate the effects of butein on inflammatory cytokines, matrix metalloproteinase-9 (MMP-9), and colitis in interleukin (IL)-10-/- mice. METHODS: To synchronize colitis, 8-to 10-wk-old IL-10-/- mice were fed pellet-chow containing piroxicam for 2 wk. Subsequently, phosphate-buffered saline or butein (1 mg/kg per day, ip) was injected for 4 wk. Histologic scores, inflammatory cytokines, MMP-9 and phosphorylated signal transducer and activator of transcription 3 (pSTAT3) expressions were analyzed in IL-10-/- mice and in Colo 205 cells. RESULTS: Butein reduced the colonic inflammatory score by > 50%. Expression levels of IL-6, IL-1β, interferon (IFN)-γ and MMP-9 were decreased in the colons of mice exposed to butein, whereas other inflammatory cytokines (IL-17A, IL-21 and IL-22) were unchanged. Immunohistochemical staining for pSTAT3 and MMP-9 was significantly decreased in the buteintreated groups compared with the controls. Butein inhibited IL-6-induced activation of STAT3 in Colo 205 cells. CONCLUSION: Butein ameliorated colitis in IL-10-/- mice by regulating IL-6/STAT3 and MMP-9 activation.

KW - Butein

KW - Colitis

KW - Inflammatory bowel disease

KW - Interleukin-6/signal transducer and activator of transcription 3

KW - Matrix metalloproteinase-9

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SN - 1007-9327

VL - 21

SP - 465

EP - 474

JO - World journal of gastroenterology

JF - World journal of gastroenterology

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ER -

Butein effects in colitis and interleukin-6/signal transducer and activator of transcription 3 expression

Abstract

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Keywords

ASJC Scopus subject areas

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