Butyric acid production with high selectivity coupled with acetic acid consumption in sugar-glycerol mixture fermentation by Clostridium tyrobutyricum ATCC25755

Hyun Ju Oh, Ki Yeon Kim, Kyung Min Lee, Sun Mi Lee, Gyeongtaek Gong, Min Kyu Oh, Youngsoon Um

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    20 Citations (Scopus)

    Abstract

    Butyric acid-producing strain Clostridium tyrobutyricum ATCC25755 produces acetic acid as a byproduct, which lowers butyric acid yield and selectivity. Supplementing glycerol would be a rational approach to enhance a NADH-requiring metabolite butyric acid selectivity by increasing NADH availability; however, there has been no report butyric acid production using glycerol as either a sole carbon source or a co-substrate with C. tyrobutyricum. In this study, glycerol was supplemented along with sugar to increase NADH availability facilitating the production of butyric acid. C. tyrobutyricum was found to simultaneously consume glucose and glycerol only when acetic acid was present. Notably, acetic acid was not produced but consumed. The amount of glycerol consumption was linearly dependent on the amount of acetic acid consumption. When mixed sugars (glucose 80 g/L and xylose 30 g/L) and crude glycerol were used, not only exogenous acetic acid consumption but also acetic acid reassimilation were clearly observed, resulting in significantly enhanced butyric acid production (54.79 g/L), yield (0.464 g/g), and selectivity (98.3%) compared to sugar-only fermentation (production, 35.42 g/L; yield, 0.339 g/g; selectivity, 69.1%). Therefore, this study would pave the way for selective and cost-effective butyric acid production using sugars and glycerol from biodiesel facility.

    Original languageEnglish
    Pages (from-to)44-51
    Number of pages8
    JournalJournal of Industrial and Engineering Chemistry
    Volume75
    DOIs
    Publication statusPublished - 2019 Jul 25

    Bibliographical note

    Funding Information:
    This research was supported by C1 Gas Refinery Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (Information & Communication Technology) ( 2016M3D3A1A01913249 ).

    Funding Information:
    This research was supported by C1 Gas Refinery Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (Information & Communication Technology) (2016M3D3A1A01913249).

    Publisher Copyright:
    © 2019 The Korean Society of Industrial and Engineering Chemistry

    Keywords

    • Acetic acid reassimilation
    • Butyric acid
    • Clostridium tyrobutyricumATCC 25755
    • Glucose
    • Glycerol

    ASJC Scopus subject areas

    • General Chemical Engineering

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