C-terminal part of AgRP stimulates insulin secretion through calcium release in pancreatic β Rin5mf cells

Soo Kim Hyeon; Sanatombi Yumkham; Hye Young Lee; Jung Hwa Cho; Mean Hwan Kim; Duk Su Koh; Ho Ryu Sung; Pann Ghill Suh

doi:10.1016/j.npep.2005.04.005

C-terminal part of AgRP stimulates insulin secretion through calcium release in pancreatic β Rin5mf cells

Soo Kim Hyeon, Sanatombi Yumkham, Hye Young Lee, Jung Hwa Cho, Mean Hwan Kim, Duk Su Koh, Ho Ryu Sung, Pann Ghill Suh

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Agouti-related protein (AgRP) is an orexigenic peptide which is composed of three parts; the amino (N)-terminus, the middle part, and the carboxyl (C)-terminus. AgRP has been implicated in various cell signaling, but the precise role of each parts are currently unclear. In this study, we have attempted to determine which part of AgRP was critical for insulin secretion. We have found that the C-terminus of AgRP specifically increases the intracellular calcium concentration in pancreatic β Rin5mf cells in a PLC-dependent manner, whereas the middle part and C-terminus have little effects on calcium release. This calcium response can be observed in the freshly isolated primary β cells also. Moreover, amperometric measurement reveals that the C-terminus of AgRP increases the rate of exocytosis in Rin5mf cells. We further show that this region of AgRP is responsible for insulin secretion in a PLC-dependent manner. Taken together, these results indicate that the C-terminus of AgRP can participate in the insulin secretion in pancreatic β cells, through the modulation of calcium release.

Original language	English
Pages (from-to)	385-393
Number of pages	9
Journal	Neuropeptides
Volume	39
Issue number	4
DOIs	https://doi.org/10.1016/j.npep.2005.04.005
Publication status	Published - 2005 Aug
Externally published	Yes

Bibliographical note

Funding Information:
This study was supported by the National R&D Program for Fusion Strategy of Advanced Technologies of Ministry of Science and Technology (MOST).

Keywords

AgRP
Calcium
Insulin
Pancreatic β cells
Phospholipase C

ASJC Scopus subject areas

Endocrinology
Neurology
Endocrine and Autonomic Systems
Cellular and Molecular Neuroscience

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10.1016/j.npep.2005.04.005

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title = "C-terminal part of AgRP stimulates insulin secretion through calcium release in pancreatic β Rin5mf cells",

abstract = "Agouti-related protein (AgRP) is an orexigenic peptide which is composed of three parts; the amino (N)-terminus, the middle part, and the carboxyl (C)-terminus. AgRP has been implicated in various cell signaling, but the precise role of each parts are currently unclear. In this study, we have attempted to determine which part of AgRP was critical for insulin secretion. We have found that the C-terminus of AgRP specifically increases the intracellular calcium concentration in pancreatic β Rin5mf cells in a PLC-dependent manner, whereas the middle part and C-terminus have little effects on calcium release. This calcium response can be observed in the freshly isolated primary β cells also. Moreover, amperometric measurement reveals that the C-terminus of AgRP increases the rate of exocytosis in Rin5mf cells. We further show that this region of AgRP is responsible for insulin secretion in a PLC-dependent manner. Taken together, these results indicate that the C-terminus of AgRP can participate in the insulin secretion in pancreatic β cells, through the modulation of calcium release.",

keywords = "AgRP, Calcium, Insulin, Pancreatic β cells, Phospholipase C",

author = "Hyeon, {Soo Kim} and Sanatombi Yumkham and Lee, {Hye Young} and Cho, {Jung Hwa} and Kim, {Mean Hwan} and Koh, {Duk Su} and Sung, {Ho Ryu} and Suh, {Pann Ghill}",

note = "Funding Information: This study was supported by the National R&D Program for Fusion Strategy of Advanced Technologies of Ministry of Science and Technology (MOST).",

year = "2005",

month = aug,

doi = "10.1016/j.npep.2005.04.005",

language = "English",

volume = "39",

pages = "385--393",

journal = "Neuropeptides",

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T1 - C-terminal part of AgRP stimulates insulin secretion through calcium release in pancreatic β Rin5mf cells

AU - Hyeon, Soo Kim

AU - Yumkham, Sanatombi

AU - Lee, Hye Young

AU - Cho, Jung Hwa

AU - Kim, Mean Hwan

AU - Koh, Duk Su

AU - Sung, Ho Ryu

AU - Suh, Pann Ghill

N1 - Funding Information: This study was supported by the National R&D Program for Fusion Strategy of Advanced Technologies of Ministry of Science and Technology (MOST).

PY - 2005/8

Y1 - 2005/8

N2 - Agouti-related protein (AgRP) is an orexigenic peptide which is composed of three parts; the amino (N)-terminus, the middle part, and the carboxyl (C)-terminus. AgRP has been implicated in various cell signaling, but the precise role of each parts are currently unclear. In this study, we have attempted to determine which part of AgRP was critical for insulin secretion. We have found that the C-terminus of AgRP specifically increases the intracellular calcium concentration in pancreatic β Rin5mf cells in a PLC-dependent manner, whereas the middle part and C-terminus have little effects on calcium release. This calcium response can be observed in the freshly isolated primary β cells also. Moreover, amperometric measurement reveals that the C-terminus of AgRP increases the rate of exocytosis in Rin5mf cells. We further show that this region of AgRP is responsible for insulin secretion in a PLC-dependent manner. Taken together, these results indicate that the C-terminus of AgRP can participate in the insulin secretion in pancreatic β cells, through the modulation of calcium release.

AB - Agouti-related protein (AgRP) is an orexigenic peptide which is composed of three parts; the amino (N)-terminus, the middle part, and the carboxyl (C)-terminus. AgRP has been implicated in various cell signaling, but the precise role of each parts are currently unclear. In this study, we have attempted to determine which part of AgRP was critical for insulin secretion. We have found that the C-terminus of AgRP specifically increases the intracellular calcium concentration in pancreatic β Rin5mf cells in a PLC-dependent manner, whereas the middle part and C-terminus have little effects on calcium release. This calcium response can be observed in the freshly isolated primary β cells also. Moreover, amperometric measurement reveals that the C-terminus of AgRP increases the rate of exocytosis in Rin5mf cells. We further show that this region of AgRP is responsible for insulin secretion in a PLC-dependent manner. Taken together, these results indicate that the C-terminus of AgRP can participate in the insulin secretion in pancreatic β cells, through the modulation of calcium release.

KW - AgRP

KW - Calcium

KW - Insulin

KW - Pancreatic β cells

KW - Phospholipase C

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ER -

C-terminal part of AgRP stimulates insulin secretion through calcium release in pancreatic β Rin5mf cells

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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