Caco-2 cell-derived biomimetic electrochemical biosensor for cholera toxin detection

Yonghwan Kim, Dongtak Lee, Youngjun Seo, Hyo Gi Jung, Jae Won Jang, Dongsung Park, Insu Kim, Jaeheung Kim, Gyudo Lee, Kyo Seon Hwang, Seung Hyun Kim, Sang Won Lee, Jeong Hoon Lee, Dae Sung Yoon

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)


Cholera is a highly contagious and lethal waterborne disease induced by an infection with Vibrio cholerae (V. cholerae) secreting cholera toxin (CTx). Cholera toxin subunit B (CTxB) from the CTx specifically binds with monosialo-tetra-hexosyl-ganglioside (GM1) found on the exterior cell membrane of an enterocyte. Bioinspired by the pathological process of CTx, we developed an electrochemical biosensor with GM1-expressing Caco-2 cell membrane (CCM) on the electrode surface. Briefly, the electrode surface was functionalized with CCM using the vesicle fusion method. We determined the CTxB detection performances of Caco-2 cell membrane-coated biosensor (CCB) using electrochemical impedance spectroscopy (EIS). the CCB had an excellent limit of detection of ∼11.46 nM and a detection range spanning 100 ng/mL - 1 mg/mL. In addition, the CCB showed high selectivity against various interfering molecules, including abundant constituents of intestinal fluid and various bacterial toxins. The long-term stability of the CCBs was also verified for 3 weeks using EIS. Overall, the CCB has excellent potential for practical use such as point-of-care and cost-effective testing for CTxB detection in developing countries.

Original languageEnglish
Article number115105
JournalBiosensors and Bioelectronics
Publication statusPublished - 2023 Apr 15

Bibliographical note

Publisher Copyright:
© 2023 Elsevier B.V.


  • Bioinspired
  • Biosensor
  • Caco-2 cell membrane
  • Cholera
  • Cholera toxin subunit B
  • Electrochemical impedance spectroscopy

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry


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