TY - JOUR
T1 - Cactus cladodes (Opuntia humifusa) extract minimizes the effects of UV irradiation on keratinocytes and hairless mice
AU - Park, Kyungmi
AU - Choi, Hyeon Son
AU - Hong, Yang Hee
AU - Jung, Eun Young
AU - Suh, Hyung Joo
N1 - Funding Information:
This research was supported by Korea University Grant (K1605441).
Funding Information:
This research was supported by Korea University Grant [K1605441].
Publisher Copyright:
© 2017 The Author(s).
PY - 2017
Y1 - 2017
N2 - Cactus cladodes [Opuntia humifusa (Raf.) Raf. (Cactaceae)] is one of the long been used as a folk medicine for skin disorders. Objective: This study investigated the skincare potential of cactus cladodes extract (OHE), including its ability to regulate ultraviolet B (UVB)-induced hyaluronic acid (HA) production. Materials and methods: Gene expression levels of hyaluronic acid synthases (HASs) and hyaluronidase (HYAL) were measured in UVB-irradiated HaCaT cells with OHE treatment (10, 25, 50, 100 µg/mL) by realtime polymerase chain reaction (PCR). The HA content was analyzed in hairless mice (SKH-1, male, 6 weeks old) treated with OHE for 10 weeks by using enzyme-linked immunosorbent assay (ELISA). Haematoxylin and eosin (H&E) and immunohistological staining were performed to examine epidermal thickness and levels of CD44 and hyaluronic acid-binding protein (HABP). Results: HA synthases (HAS,1 HAS2, HAS3) mRNA levels were increased by 1.9-, 2.2- and 1.6-fold, respectively, with OHE treatment (100 µg/mL), while UVB-induced increase of hyaluronidase mRNA significantly decreased by 35%. HA content in animal was decreased from 42.9 to 27.1 ng/mL by OHE treatment. HAS mRNA levels were decreased by 39%, but HYAL mRNA was increased by 50% in OHE group. CD44 and HABP levels, which were greatly increased by UVB-irradiation, were reduced by 64 and 60%, respectively. Epidermal thickness, transepidermal water loss (TEWL), and erythema formation was also decreased by 45 (45.7 to 24.2 µm), 48 (48.8 to 25 g/h/m2) and 33%, respectively. Conclusion: OHE protects skin from UVB-induced skin degeneration in HaCaT cells and hairless mice.
AB - Cactus cladodes [Opuntia humifusa (Raf.) Raf. (Cactaceae)] is one of the long been used as a folk medicine for skin disorders. Objective: This study investigated the skincare potential of cactus cladodes extract (OHE), including its ability to regulate ultraviolet B (UVB)-induced hyaluronic acid (HA) production. Materials and methods: Gene expression levels of hyaluronic acid synthases (HASs) and hyaluronidase (HYAL) were measured in UVB-irradiated HaCaT cells with OHE treatment (10, 25, 50, 100 µg/mL) by realtime polymerase chain reaction (PCR). The HA content was analyzed in hairless mice (SKH-1, male, 6 weeks old) treated with OHE for 10 weeks by using enzyme-linked immunosorbent assay (ELISA). Haematoxylin and eosin (H&E) and immunohistological staining were performed to examine epidermal thickness and levels of CD44 and hyaluronic acid-binding protein (HABP). Results: HA synthases (HAS,1 HAS2, HAS3) mRNA levels were increased by 1.9-, 2.2- and 1.6-fold, respectively, with OHE treatment (100 µg/mL), while UVB-induced increase of hyaluronidase mRNA significantly decreased by 35%. HA content in animal was decreased from 42.9 to 27.1 ng/mL by OHE treatment. HAS mRNA levels were decreased by 39%, but HYAL mRNA was increased by 50% in OHE group. CD44 and HABP levels, which were greatly increased by UVB-irradiation, were reduced by 64 and 60%, respectively. Epidermal thickness, transepidermal water loss (TEWL), and erythema formation was also decreased by 45 (45.7 to 24.2 µm), 48 (48.8 to 25 g/h/m2) and 33%, respectively. Conclusion: OHE protects skin from UVB-induced skin degeneration in HaCaT cells and hairless mice.
KW - CD44
KW - Ha-binding protein
KW - Hacat cell
KW - Hyaluronic acid synthase
KW - Hyaluronidase
KW - SKH-1 mice
UR - http://www.scopus.com/inward/record.url?scp=85014950079&partnerID=8YFLogxK
U2 - 10.1080/13880209.2017.1286357
DO - 10.1080/13880209.2017.1286357
M3 - Article
C2 - 28183235
AN - SCOPUS:85014950079
SN - 1388-0209
VL - 55
SP - 1032
EP - 1040
JO - Pharmaceutical Biology
JF - Pharmaceutical Biology
IS - 1
ER -