Calcium imaging in gentamicin ototoxicity: Increased intracellular calcium relates to oxidative stress and late apoptosis

Jiwon Chang; Ji Yun Yang; June Choi; Hak Hyun Jung; Gi Jung Im

doi:10.1016/j.ijporl.2011.09.027

Calcium imaging in gentamicin ototoxicity: Increased intracellular calcium relates to oxidative stress and late apoptosis

Jiwon Chang, Ji Yun Yang, June Choi, Hak Hyun Jung, Gi Jung Im

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

Objectives: To estimate intracellular calcium changes in gentamicin (GM) ototoxicity using calcium imaging. To investigate GM-induced physiologic changes in auditory cells including cell viability, apoptosis, and oxidative stress. Methods: Varying concentrations of GM were applied to the HEI-OC1 cochlear cell line. Calcium imaging tracked changes in intracellular calcium concentration during GM cytotoxicity. Cell viability and intracellular reactive oxygen species (ROS) levels also were measured. Results: Little change in calcium levels occurred in HEI-OC1 cells exposed to less than 35. mM GM. However, calcium rose continuously in cells exposed to more than 60. mM GM. With administration of intermediate concentrations of 40 or 50. mM GM, calcium increased variably in different cells, returning to baseline in some cases, or rising continuously in others. Upon increase of GM concentration, intracellular calcium concentration and ROS were increased, and cell viability was decreased due to late apoptosis. Conclusion: This study shows that GM increased intracellular calcium, ROS, and late apoptosis of HEI-OC1 cells derived from cochlear tissue. Increase of intracellular calcium is related to GM-induced apoptosis and oxidative stress. Calcium imaging can be used to determine change of intracellular calcium concentrations and apoptosis in GM ototoxicity.

Original language	English
Pages (from-to)	1616-1622
Number of pages	7
Journal	International Journal of Pediatric Otorhinolaryngology
Volume	75
Issue number	12
DOIs	https://doi.org/10.1016/j.ijporl.2011.09.027
Publication status	Published - 2011 Dec
Externally published	Yes

Bibliographical note

Funding Information:
Thanks to Paul Albert Fuchs, Professor of Johns Hopkins University School of Medicine, for kind advice and editing. This work was supported by the National Research Foundation of Korea Grant funded by the Korean Government MEST, Basic Research Promotion Fund ( NRF-2010-013-E00015 ), and the Korea Research Foundation Grant funded by the Korean Government (MOEHRD, Basic Research Promotion Fund) ( KRF-2009-0066388 ). This funding source provides only financial support and plays no specific scientific role in this study.

Keywords

Apoptosis
Calcium
Cochlea
Fura-2
Gentamicins
Meniere's disease
Reactive oxygen species

ASJC Scopus subject areas

Pediatrics, Perinatology, and Child Health
Otorhinolaryngology

Access to Document

10.1016/j.ijporl.2011.09.027

Cite this

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title = "Calcium imaging in gentamicin ototoxicity: Increased intracellular calcium relates to oxidative stress and late apoptosis",

abstract = "Objectives: To estimate intracellular calcium changes in gentamicin (GM) ototoxicity using calcium imaging. To investigate GM-induced physiologic changes in auditory cells including cell viability, apoptosis, and oxidative stress. Methods: Varying concentrations of GM were applied to the HEI-OC1 cochlear cell line. Calcium imaging tracked changes in intracellular calcium concentration during GM cytotoxicity. Cell viability and intracellular reactive oxygen species (ROS) levels also were measured. Results: Little change in calcium levels occurred in HEI-OC1 cells exposed to less than 35. mM GM. However, calcium rose continuously in cells exposed to more than 60. mM GM. With administration of intermediate concentrations of 40 or 50. mM GM, calcium increased variably in different cells, returning to baseline in some cases, or rising continuously in others. Upon increase of GM concentration, intracellular calcium concentration and ROS were increased, and cell viability was decreased due to late apoptosis. Conclusion: This study shows that GM increased intracellular calcium, ROS, and late apoptosis of HEI-OC1 cells derived from cochlear tissue. Increase of intracellular calcium is related to GM-induced apoptosis and oxidative stress. Calcium imaging can be used to determine change of intracellular calcium concentrations and apoptosis in GM ototoxicity.",

keywords = "Apoptosis, Calcium, Cochlea, Fura-2, Gentamicins, Meniere's disease, Reactive oxygen species",

author = "Jiwon Chang and Yang, {Ji Yun} and June Choi and Jung, {Hak Hyun} and Im, {Gi Jung}",

note = "Funding Information: Thanks to Paul Albert Fuchs, Professor of Johns Hopkins University School of Medicine, for kind advice and editing. This work was supported by the National Research Foundation of Korea Grant funded by the Korean Government MEST, Basic Research Promotion Fund ( NRF-2010-013-E00015 ), and the Korea Research Foundation Grant funded by the Korean Government (MOEHRD, Basic Research Promotion Fund) ( KRF-2009-0066388 ). This funding source provides only financial support and plays no specific scientific role in this study.",

year = "2011",

month = dec,

doi = "10.1016/j.ijporl.2011.09.027",

language = "English",

volume = "75",

pages = "1616--1622",

journal = "International Journal of Pediatric Otorhinolaryngology",

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T1 - Calcium imaging in gentamicin ototoxicity

T2 - Increased intracellular calcium relates to oxidative stress and late apoptosis

AU - Chang, Jiwon

AU - Yang, Ji Yun

AU - Choi, June

AU - Jung, Hak Hyun

AU - Im, Gi Jung

N1 - Funding Information: Thanks to Paul Albert Fuchs, Professor of Johns Hopkins University School of Medicine, for kind advice and editing. This work was supported by the National Research Foundation of Korea Grant funded by the Korean Government MEST, Basic Research Promotion Fund ( NRF-2010-013-E00015 ), and the Korea Research Foundation Grant funded by the Korean Government (MOEHRD, Basic Research Promotion Fund) ( KRF-2009-0066388 ). This funding source provides only financial support and plays no specific scientific role in this study.

PY - 2011/12

Y1 - 2011/12

N2 - Objectives: To estimate intracellular calcium changes in gentamicin (GM) ototoxicity using calcium imaging. To investigate GM-induced physiologic changes in auditory cells including cell viability, apoptosis, and oxidative stress. Methods: Varying concentrations of GM were applied to the HEI-OC1 cochlear cell line. Calcium imaging tracked changes in intracellular calcium concentration during GM cytotoxicity. Cell viability and intracellular reactive oxygen species (ROS) levels also were measured. Results: Little change in calcium levels occurred in HEI-OC1 cells exposed to less than 35. mM GM. However, calcium rose continuously in cells exposed to more than 60. mM GM. With administration of intermediate concentrations of 40 or 50. mM GM, calcium increased variably in different cells, returning to baseline in some cases, or rising continuously in others. Upon increase of GM concentration, intracellular calcium concentration and ROS were increased, and cell viability was decreased due to late apoptosis. Conclusion: This study shows that GM increased intracellular calcium, ROS, and late apoptosis of HEI-OC1 cells derived from cochlear tissue. Increase of intracellular calcium is related to GM-induced apoptosis and oxidative stress. Calcium imaging can be used to determine change of intracellular calcium concentrations and apoptosis in GM ototoxicity.

AB - Objectives: To estimate intracellular calcium changes in gentamicin (GM) ototoxicity using calcium imaging. To investigate GM-induced physiologic changes in auditory cells including cell viability, apoptosis, and oxidative stress. Methods: Varying concentrations of GM were applied to the HEI-OC1 cochlear cell line. Calcium imaging tracked changes in intracellular calcium concentration during GM cytotoxicity. Cell viability and intracellular reactive oxygen species (ROS) levels also were measured. Results: Little change in calcium levels occurred in HEI-OC1 cells exposed to less than 35. mM GM. However, calcium rose continuously in cells exposed to more than 60. mM GM. With administration of intermediate concentrations of 40 or 50. mM GM, calcium increased variably in different cells, returning to baseline in some cases, or rising continuously in others. Upon increase of GM concentration, intracellular calcium concentration and ROS were increased, and cell viability was decreased due to late apoptosis. Conclusion: This study shows that GM increased intracellular calcium, ROS, and late apoptosis of HEI-OC1 cells derived from cochlear tissue. Increase of intracellular calcium is related to GM-induced apoptosis and oxidative stress. Calcium imaging can be used to determine change of intracellular calcium concentrations and apoptosis in GM ototoxicity.

KW - Apoptosis

KW - Calcium

KW - Cochlea

KW - Fura-2

KW - Gentamicins

KW - Meniere's disease

KW - Reactive oxygen species

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Calcium imaging in gentamicin ototoxicity: Increased intracellular calcium relates to oxidative stress and late apoptosis

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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