CaLecRK-S.5, a pepper L-type lectin receptor kinase gene, accelerates Phytophthora elicitin-mediated defense response

Joo Yong Woo; Young Jin Kim; Kyung Hee Paek

doi:10.1016/j.bbrc.2020.02.014

CaLecRK-S.5, a pepper L-type lectin receptor kinase gene, accelerates Phytophthora elicitin-mediated defense response

Joo Yong Woo
, Young Jin Kim
, Kyung Hee Paek^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

Innate immunity in plants relies on the recognition of pathogen-associated molecular patterns (PAMPs) by pattern-recognition receptors (PRRs) located on the plant cell surface. CaLecRK-S.5, a pepper L-type lectin receptor kinase, has been shown to confer broad-spectrum resistance through priming activation. To further elucidate the molecular mechanism of CaLecRK-S.5, transgenic tobacco plants were generated in this study. Interestingly, hemizygous transgenic plants exhibited a high accumulation of CaLecRK-S.5, but this accumulation was completely abolished in homozygous transgenic plants by a cosuppression mechanism. Gain-of-function and loss-of-function analyses revealed that CaLecRK-S.5 plays a positive role in Phytophthora elicitin-mediated defense responses.

Original language	English
Pages (from-to)	951-956
Number of pages	6
Journal	Biochemical and biophysical research communications
Volume	524
Issue number	4
DOIs	https://doi.org/10.1016/j.bbrc.2020.02.014
Publication status	Published - 2020 Apr 16

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government ( MSIP ) (No. R1A2A2A01002476 ) and a Korea University Grant, Republic of Korea .

Publisher Copyright:
© 2020 Elsevier Inc.

Keywords

Cosuppression mechanism
INF1
L-type lectin receptor kinase
Pattern-recognition receptors (PRRs)

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2020.02.014

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title = "CaLecRK-S.5, a pepper L-type lectin receptor kinase gene, accelerates Phytophthora elicitin-mediated defense response",

abstract = "Innate immunity in plants relies on the recognition of pathogen-associated molecular patterns (PAMPs) by pattern-recognition receptors (PRRs) located on the plant cell surface. CaLecRK-S.5, a pepper L-type lectin receptor kinase, has been shown to confer broad-spectrum resistance through priming activation. To further elucidate the molecular mechanism of CaLecRK-S.5, transgenic tobacco plants were generated in this study. Interestingly, hemizygous transgenic plants exhibited a high accumulation of CaLecRK-S.5, but this accumulation was completely abolished in homozygous transgenic plants by a cosuppression mechanism. Gain-of-function and loss-of-function analyses revealed that CaLecRK-S.5 plays a positive role in Phytophthora elicitin-mediated defense responses.",

keywords = "Cosuppression mechanism, INF1, L-type lectin receptor kinase, Pattern-recognition receptors (PRRs)",

author = "Woo, \{Joo Yong\} and Kim, \{Young Jin\} and Paek, \{Kyung Hee\}",

note = "Funding Information: This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government ( MSIP ) (No. R1A2A2A01002476 ) and a Korea University Grant, Republic of Korea . Publisher Copyright: {\textcopyright} 2020 Elsevier Inc.",

year = "2020",

month = apr,

day = "16",

doi = "10.1016/j.bbrc.2020.02.014",

language = "English",

volume = "524",

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T1 - CaLecRK-S.5, a pepper L-type lectin receptor kinase gene, accelerates Phytophthora elicitin-mediated defense response

AU - Woo, Joo Yong

AU - Kim, Young Jin

AU - Paek, Kyung Hee

N1 - Funding Information: This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government ( MSIP ) (No. R1A2A2A01002476 ) and a Korea University Grant, Republic of Korea . Publisher Copyright: © 2020 Elsevier Inc.

PY - 2020/4/16

Y1 - 2020/4/16

N2 - Innate immunity in plants relies on the recognition of pathogen-associated molecular patterns (PAMPs) by pattern-recognition receptors (PRRs) located on the plant cell surface. CaLecRK-S.5, a pepper L-type lectin receptor kinase, has been shown to confer broad-spectrum resistance through priming activation. To further elucidate the molecular mechanism of CaLecRK-S.5, transgenic tobacco plants were generated in this study. Interestingly, hemizygous transgenic plants exhibited a high accumulation of CaLecRK-S.5, but this accumulation was completely abolished in homozygous transgenic plants by a cosuppression mechanism. Gain-of-function and loss-of-function analyses revealed that CaLecRK-S.5 plays a positive role in Phytophthora elicitin-mediated defense responses.

AB - Innate immunity in plants relies on the recognition of pathogen-associated molecular patterns (PAMPs) by pattern-recognition receptors (PRRs) located on the plant cell surface. CaLecRK-S.5, a pepper L-type lectin receptor kinase, has been shown to confer broad-spectrum resistance through priming activation. To further elucidate the molecular mechanism of CaLecRK-S.5, transgenic tobacco plants were generated in this study. Interestingly, hemizygous transgenic plants exhibited a high accumulation of CaLecRK-S.5, but this accumulation was completely abolished in homozygous transgenic plants by a cosuppression mechanism. Gain-of-function and loss-of-function analyses revealed that CaLecRK-S.5 plays a positive role in Phytophthora elicitin-mediated defense responses.

KW - Cosuppression mechanism

KW - INF1

KW - L-type lectin receptor kinase

KW - Pattern-recognition receptors (PRRs)

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DO - 10.1016/j.bbrc.2020.02.014

M3 - Article

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AN - SCOPUS:85079274032

SN - 0006-291X

VL - 524

SP - 951

EP - 956

JO - Biochemical and biophysical research communications

JF - Biochemical and biophysical research communications

IS - 4

ER -

CaLecRK-S.5, a pepper L-type lectin receptor kinase gene, accelerates Phytophthora elicitin-mediated defense response

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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