CaLecRK-S.5, a pepper L-type lectin receptor kinase gene, confers broad-spectrum resistance by activating priming

Joo Yong Woo, Kwang Ju Jeong, Young Jin Kim, Kyung Hee Paek

    Research output: Contribution to journalArticlepeer-review

    24 Citations (Scopus)

    Abstract

    In Arabidopsis, several L-type lectin receptor kinases (LecRKs) have been identified as putative immune receptors. However, to date, there have been few analyses of LecRKs in crop plants. Virus-induced gene silencing of CaLecRK-S.5 verified the role of CaLecRK-S.5 in broad-spectrum resistance. Compared with control plants, CaLecRK-S.5-silenced plants showed reduced hypersensitive response, reactive oxygen species burst, secondary metabolite production, mitogen-activated protein kinase activation, and defense-related gene expression in response to Tobacco mosaic virus pathotype P0 (TMV-P0) infection. Suppression of CaLecRK-S.5 expression significantly enhanced the susceptibility to Pepper mild mottle virus pathotype P1,2,3, Xanthomonas campestris pv. vesicatoria, Phytophthora capsici, as well as TMV-P0. Additionally, β-aminobutyric acid treatment and a systemic acquired resistance assay revealed that CaLecRK-S.5 is involved in priming of plant immunity. Pre-treatment with β-aminobutyric acid before viral infection restored the reduced disease resistance phenotypes shown in CaLecRK-S.5-silenced plants. Systemic acquired resistance was also abolished in CaLecRK-S.5-silenced plants. Finally, RNA sequencing analysis indicated that CaLecRK-S.5 positively regulates plant immunity at the transcriptional level. Altogether, these results suggest that CaLecRK-S.5-mediated broad-spectrum resistance is associated with the regulation of priming.

    Original languageEnglish
    Pages (from-to)5725-5741
    Number of pages17
    JournalJournal of experimental botany
    Volume67
    Issue number19
    DOIs
    Publication statusPublished - 2016 Oct 1

    Bibliographical note

    Publisher Copyright:
    © 2016 The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology.

    Keywords

    • Broad-spectrum resistance
    • CaLecRK-S.5
    • L-type lectin receptor kinase
    • RNA sequencing
    • SAR
    • priming
    • tobamovirus

    ASJC Scopus subject areas

    • Physiology
    • Plant Science

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