Objective Celastrol, a triterpene from the root bark of the Chinese medicinal plant Tripterygium wilfordii, has been shown to exhibit anti-oxidant, anti-inflammatory, anti-cancer and insecticidal activities. Also, it has been demonstrated that celastrol has obesity-controlling effects in diet-induced obesity mice. However, direct evidence that celastrol contributes to the development of adipocyte differentiation and lipolysis has not been fully elucidated. Moreover, no previous studies have evaluated whether celastrol may regulate adipogenic transcriptional markers in adipocytes. Materials/Methods In order to address the questions above, we extended previous observations and investigated in vitro celastrol signaling study whether celastrol may regulate differentiation, lipolysis and key adipogenic transcriptional pathways in 3T3-L1 adipocytes. Results Treatment of celastrol not only inhibited adipocyte differentiation (lipid accumulation, glyceraldehyde-3-phosphate dehydrogenase activity and triglyceride content) but also increased lipolysis (glycerol release and free fatty acid release) in 3T3-L1 adipocytes. In addition, all celastrol-regulated functional activities were controlled by PPARγ2 and C/EBPα signaling pathways in duration of celastrol's treatment in 3T3-L1 adipocytes. Conclusion Our initial data from in vitro celastrol signaling studies suggest novel insights into the role of PPARγ2 and C/EBPα as probable mediators of the action of celastrol in regulating adipocyte differentiation and lipolysis in 3T3-L1 adipocytes.
Bibliographical noteFunding Information:
This work was supported by a Korea University Grant ( K142078 and K1515281 ), a Korea University Guro Hospital Grant ( O1500081 ), and a Korea Institute of Oriental Medicine Grant ( R1508541 ). Also, this research was supported by Basic Research Program through the National Research Foundation of Korea ( NRF 2015R1D1A1A01056762 ) funded by the Ministry of Education and was supported by Bio & Medical Technology Development Program through the National Research Foundation of Korea funded by the Ministry of Education, Science and Technology ( NRF 2015M3A9B4071075 ).
© 2016 Elsevier Inc. All rights reserved.
- Adipocytes differentiation
ASJC Scopus subject areas
- Endocrinology, Diabetes and Metabolism