C─C motif chemokine ligand 2 regulates lps-induced inflammation and ER stress to enhance proliferation of bovine endometrial epithelial cells

Whasun Lim; Hyocheol Bae; Fuller W. Bazer; Sung Man Kim; Gwonhwa Song

doi:10.1002/jcp.26151

C─C motif chemokine ligand 2 regulates lps-induced inflammation and ER stress to enhance proliferation of bovine endometrial epithelial cells

Whasun Lim, Hyocheol Bae, Fuller W. Bazer, Sung Man Kim, Gwonhwa Song

Department of Biotechnology

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Chemokines play an important role in regulating the complex immune system at the maternal-fetal interface during pregnancy. Among various chemokines, C─C motif chemokine ligand 2 (CCL2) plays a role in the recruitment of immune regulatory cells to implantation sites within the endometrium. In cattle, CCL2 is abundantly expressed in the uterine endometrium. However, its intracellular signaling has not been identified. In this study, we examined the effects of CCL2 on bovine endometrial (BEND) cell proliferation. CCL2 stimulated BEND cell proliferation by abundant expression of PCNA, accumulation of cells in the G2/M phase, and activation of the PI3 K/AKT and MAPK signaling pathways. Moreover, CCL2 reduced endoplasmic reticulum stress and restored the inflammation-induced reduction in BEND cell proliferation by regulating the unfolded protein response genes and cytokines. Collectively, these results demonstrated that CCL2 plays a pivotal role in reproductive tissues and may support maternal-fetal interface to improve efficiency of pregnancy.

Original language	English
Pages (from-to)	3141-3151
Number of pages	11
Journal	Journal of Cellular Physiology
Volume	233
Issue number	4
DOIs	https://doi.org/10.1002/jcp.26151
Publication status	Published - 2018 Apr

Bibliographical note

Funding Information:
This research was funded by Basic Science Research Program (2015R1D1A1A01059331) through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science, and Technology. This research was supported by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (grant number: HI17C0929).

Funding Information:
Ministry of Education, Science, and Technology (Republic of Korea), Grant number: 2015R1D1A1A01059331; Ministry of Health & Welfare, Republic of Korea (Republic of Korea), Grant number: HI17C0929

Publisher Copyright:
© 2017 Wiley Periodicals, Inc.

Keywords

CCL2
Endometrial epithelial cells
Endoplasmic reticulum stress
Immune system

ASJC Scopus subject areas

Physiology
Clinical Biochemistry
Cell Biology

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10.1002/jcp.26151

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title = "C─C motif chemokine ligand 2 regulates lps-induced inflammation and ER stress to enhance proliferation of bovine endometrial epithelial cells",

abstract = "Chemokines play an important role in regulating the complex immune system at the maternal-fetal interface during pregnancy. Among various chemokines, C─C motif chemokine ligand 2 (CCL2) plays a role in the recruitment of immune regulatory cells to implantation sites within the endometrium. In cattle, CCL2 is abundantly expressed in the uterine endometrium. However, its intracellular signaling has not been identified. In this study, we examined the effects of CCL2 on bovine endometrial (BEND) cell proliferation. CCL2 stimulated BEND cell proliferation by abundant expression of PCNA, accumulation of cells in the G2/M phase, and activation of the PI3 K/AKT and MAPK signaling pathways. Moreover, CCL2 reduced endoplasmic reticulum stress and restored the inflammation-induced reduction in BEND cell proliferation by regulating the unfolded protein response genes and cytokines. Collectively, these results demonstrated that CCL2 plays a pivotal role in reproductive tissues and may support maternal-fetal interface to improve efficiency of pregnancy.",

keywords = "CCL2, Endometrial epithelial cells, Endoplasmic reticulum stress, Immune system",

author = "Whasun Lim and Hyocheol Bae and Bazer, {Fuller W.} and Kim, {Sung Man} and Gwonhwa Song",

note = "Funding Information: This research was funded by Basic Science Research Program (2015R1D1A1A01059331) through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science, and Technology. This research was supported by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (grant number: HI17C0929). Funding Information: Ministry of Education, Science, and Technology (Republic of Korea), Grant number: 2015R1D1A1A01059331; Ministry of Health & Welfare, Republic of Korea (Republic of Korea), Grant number: HI17C0929 Publisher Copyright: {\textcopyright} 2017 Wiley Periodicals, Inc.",

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AU - Song, Gwonhwa

N1 - Funding Information: This research was funded by Basic Science Research Program (2015R1D1A1A01059331) through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science, and Technology. This research was supported by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (grant number: HI17C0929). Funding Information: Ministry of Education, Science, and Technology (Republic of Korea), Grant number: 2015R1D1A1A01059331; Ministry of Health & Welfare, Republic of Korea (Republic of Korea), Grant number: HI17C0929 Publisher Copyright: © 2017 Wiley Periodicals, Inc.

PY - 2018/4

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N2 - Chemokines play an important role in regulating the complex immune system at the maternal-fetal interface during pregnancy. Among various chemokines, C─C motif chemokine ligand 2 (CCL2) plays a role in the recruitment of immune regulatory cells to implantation sites within the endometrium. In cattle, CCL2 is abundantly expressed in the uterine endometrium. However, its intracellular signaling has not been identified. In this study, we examined the effects of CCL2 on bovine endometrial (BEND) cell proliferation. CCL2 stimulated BEND cell proliferation by abundant expression of PCNA, accumulation of cells in the G2/M phase, and activation of the PI3 K/AKT and MAPK signaling pathways. Moreover, CCL2 reduced endoplasmic reticulum stress and restored the inflammation-induced reduction in BEND cell proliferation by regulating the unfolded protein response genes and cytokines. Collectively, these results demonstrated that CCL2 plays a pivotal role in reproductive tissues and may support maternal-fetal interface to improve efficiency of pregnancy.

AB - Chemokines play an important role in regulating the complex immune system at the maternal-fetal interface during pregnancy. Among various chemokines, C─C motif chemokine ligand 2 (CCL2) plays a role in the recruitment of immune regulatory cells to implantation sites within the endometrium. In cattle, CCL2 is abundantly expressed in the uterine endometrium. However, its intracellular signaling has not been identified. In this study, we examined the effects of CCL2 on bovine endometrial (BEND) cell proliferation. CCL2 stimulated BEND cell proliferation by abundant expression of PCNA, accumulation of cells in the G2/M phase, and activation of the PI3 K/AKT and MAPK signaling pathways. Moreover, CCL2 reduced endoplasmic reticulum stress and restored the inflammation-induced reduction in BEND cell proliferation by regulating the unfolded protein response genes and cytokines. Collectively, these results demonstrated that CCL2 plays a pivotal role in reproductive tissues and may support maternal-fetal interface to improve efficiency of pregnancy.

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C─C motif chemokine ligand 2 regulates lps-induced inflammation and ER stress to enhance proliferation of bovine endometrial epithelial cells

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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