Cd 2+ binds to Atx1 and affects the physical interaction between Atx1 and Ccc2 in Saccharomyces cerevisiae

Dong Hyuk Heo; In Joon Baek; Hyun Jun Kang; Ji Hyun Kim; Miwha Chang; Chang Min Kang; Cheol Won Yun

doi:10.1007/s10529-011-0763-9

Cd ²⁺ binds to Atx1 and affects the physical interaction between Atx1 and Ccc2 in Saccharomyces cerevisiae

Dong Hyuk Heo, In Joon Baek, Hyun Jun Kang, Ji Hyun Kim, Miwha Chang, Chang Min Kang, Cheol Won Yun

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

The ATX1 deletion strain of Saccharomyces cerevisiae is more resistant to Cd ²⁺ than the wild-type. To investigate the function of Atx1 in Cd ²⁺ toxicity, we used a metal-binding assay to study the interaction between Atx1 and Cd ²⁺ in vitro. Using circular dichroism and two-hybrid analyses, we found that Atx1 can bind Cd ²⁺ specifically and that Cd ²⁺ binding to Atx1 affects the physical interaction between Atx1 and Ccc2. These results imply that Atx1 delivers Cd ²⁺ to Ccc2 and that this delivery is, at least in part, responsible for Cd ²⁺ toxicity in S. cerevisiae.

Original language	English
Pages (from-to)	303-307
Number of pages	5
Journal	Biotechnology letters
Volume	34
Issue number	2
DOIs	https://doi.org/10.1007/s10529-011-0763-9
Publication status	Published - 2012 Feb

Bibliographical note

Funding Information:
Acknowledgment This work was supported by the Korea Ministry of the Environment as ‘‘The GAIA Project’’.

Copyright:
Copyright 2012 Elsevier B.V., All rights reserved.

Keywords

Atx1
Cadmium
Ccc2
Copper
Saccharomyces cerevisiae

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

Access to Document

10.1007/s10529-011-0763-9

Cite this

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title = "Cd 2+ binds to Atx1 and affects the physical interaction between Atx1 and Ccc2 in Saccharomyces cerevisiae",

abstract = "The ATX1 deletion strain of Saccharomyces cerevisiae is more resistant to Cd 2+ than the wild-type. To investigate the function of Atx1 in Cd 2+ toxicity, we used a metal-binding assay to study the interaction between Atx1 and Cd 2+ in vitro. Using circular dichroism and two-hybrid analyses, we found that Atx1 can bind Cd 2+ specifically and that Cd 2+ binding to Atx1 affects the physical interaction between Atx1 and Ccc2. These results imply that Atx1 delivers Cd 2+ to Ccc2 and that this delivery is, at least in part, responsible for Cd 2+ toxicity in S. cerevisiae.",

keywords = "Atx1, Cadmium, Ccc2, Copper, Saccharomyces cerevisiae",

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note = "Funding Information: Acknowledgment This work was supported by the Korea Ministry of the Environment as {\textquoteleft}{\textquoteleft}The GAIA Project{\textquoteright}{\textquoteright}. Copyright: Copyright 2012 Elsevier B.V., All rights reserved.",

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AU - Heo, Dong Hyuk

AU - Baek, In Joon

AU - Kang, Hyun Jun

AU - Kim, Ji Hyun

AU - Chang, Miwha

AU - Kang, Chang Min

AU - Yun, Cheol Won

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AB - The ATX1 deletion strain of Saccharomyces cerevisiae is more resistant to Cd 2+ than the wild-type. To investigate the function of Atx1 in Cd 2+ toxicity, we used a metal-binding assay to study the interaction between Atx1 and Cd 2+ in vitro. Using circular dichroism and two-hybrid analyses, we found that Atx1 can bind Cd 2+ specifically and that Cd 2+ binding to Atx1 affects the physical interaction between Atx1 and Ccc2. These results imply that Atx1 delivers Cd 2+ to Ccc2 and that this delivery is, at least in part, responsible for Cd 2+ toxicity in S. cerevisiae.

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