CD4+/CD8+ Ratio and Growth Differentiation Factor 8 Levels in Peripheral Blood of Large Canine Males Are Useful Parameters to Build an Age Prediction Model

Han Jun Lee, Seok Jin Hong, Seung Soo Kim, Young Yon Kwon, Bong Hwan Choi, Kyung Mi Choi, Seo Hyeong Sheen, Myung Jin Lee, Sun Young Hwang, Kyoungwan Park, Younghun Joo, Hwayoung Song, Cheol Koo Lee

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


Purpose: To build an age prediction model, we measured CD4+ and CD8+ cells, and humoral components in canine peripheral blood. Materials and Methods: Large Belgian Malinois (BGM) and German Shepherd Dog (GSD) breeds (n=27), aged from 1 to 12 years, were used for this study. Peripheral bloods were obtained by venepuncture, then plasma and peripheral blood mononuclear cells (PBMCs) were separated immediately. Six myokines, including interleukin (IL)-6, IL-8, IL-15, leukemia inhibitory factor (LIF), growth differentiation factor 8 (GDF8), and GDF11 were measured from plasma and CD4+/CD8+ T-lymphocytes ratio were measured from PBMC. These parameters were then tested with age prediction models to find the best fit model. Results: we found that the T-lymphocyte ratio (CD4+/CD8+) was significantly correlated with age (r=0.46, p=0.016). Among the six myokines, only GDF8 showed a significant correlation with age (r=0.52, p=0.005). Interestingly, these two markers showed better correlations in male dogs than females, and BGM breed than GSD. Using these two age biomarkers, we could obtain the best fit in a quadratic linear mixed model (r=0.77, p=3×10-6). Conclusions: Age prediction is a challenging task because of complication with biological age. Our quadratic linear mixed model using CD4+/CD8+ ratio and GDF8 level showed a meaningful age prediction.

Original languageEnglish
JournalWorld Journal of Men?s Health
Publication statusPublished - 2022

Bibliographical note

Funding Information:
This work was supported by the Cooperative Research Program for the Agriculture Science & Technology Development under Grant [Project No. PJ01195001], Rural Development Administration, Republic of Korea, Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2020R1F1A1075785), and a Korea University Grant.

Publisher Copyright:
Copyright © 2022 Korean Society for Sexual Medicine and Andrology


  • Age prediction model
  • Aging
  • CD4-CD8 ratio
  • Dogs
  • Myostatin

ASJC Scopus subject areas

  • Reproductive Medicine
  • Ageing
  • Health Policy
  • Public Health, Environmental and Occupational Health
  • Psychiatry and Mental health
  • Urology
  • Pharmacology (medical)


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