Cell damage during femtosecond optical trapping

Kang Bin Im; Sumin Han; Hwajoon Park; Dan Jin; Soo Kie Kim; Beop Min Kim

doi:10.1117/12.591446

Cell damage during femtosecond optical trapping

Kang Bin Im, Sumin Han, Hwajoon Park, Dan Jin, Soo Kie Kim, Beop Min Kim

Research output: Contribution to journal › Conference article › peer-review

2 Citations (Scopus)

Abstract

Microparticles including biological cells were trapped and manipulated using a continuous wave tweezer and a femtosecond laser tweezer. The difference of the optical trapping force between CW and femtosecond optical tweezers increased as the particle size decreased, possibly due to the self-focusing effect of the ultrashort pulses. Also, the white damage spots were generated near the focus during femtosecond optical trapping of biological cells even with extremely low average power. The instantaneous optical damage threshold was measured as a function of the trap depth as well. These results may help to optimize the optical trapping of biological cells using femtosecond lasers.

Original language	English
Article number	40
Pages (from-to)	274-280
Number of pages	7
Journal	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	5699
DOIs	https://doi.org/10.1117/12.591446
Publication status	Published - 2005
Externally published	Yes
Event	Imaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals and Applications III - San Jose, CA, United States Duration: 2005 Jan 24 → 2005 Jan 27

Keywords

Femtosecond lasers
Laser damage
Optical trapping
Tweezers

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Biomaterials
Atomic and Molecular Physics, and Optics
Radiology Nuclear Medicine and imaging

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10.1117/12.591446

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title = "Cell damage during femtosecond optical trapping",

abstract = "Microparticles including biological cells were trapped and manipulated using a continuous wave tweezer and a femtosecond laser tweezer. The difference of the optical trapping force between CW and femtosecond optical tweezers increased as the particle size decreased, possibly due to the self-focusing effect of the ultrashort pulses. Also, the white damage spots were generated near the focus during femtosecond optical trapping of biological cells even with extremely low average power. The instantaneous optical damage threshold was measured as a function of the trap depth as well. These results may help to optimize the optical trapping of biological cells using femtosecond lasers.",

keywords = "Femtosecond lasers, Laser damage, Optical trapping, Tweezers",

author = "Im, {Kang Bin} and Sumin Han and Hwajoon Park and Dan Jin and Kim, {Soo Kie} and Kim, {Beop Min}",

year = "2005",

doi = "10.1117/12.591446",

language = "English",

volume = "5699",

pages = "274--280",

journal = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

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TY - JOUR

T1 - Cell damage during femtosecond optical trapping

AU - Im, Kang Bin

AU - Han, Sumin

AU - Park, Hwajoon

AU - Jin, Dan

AU - Kim, Soo Kie

AU - Kim, Beop Min

PY - 2005

Y1 - 2005

N2 - Microparticles including biological cells were trapped and manipulated using a continuous wave tweezer and a femtosecond laser tweezer. The difference of the optical trapping force between CW and femtosecond optical tweezers increased as the particle size decreased, possibly due to the self-focusing effect of the ultrashort pulses. Also, the white damage spots were generated near the focus during femtosecond optical trapping of biological cells even with extremely low average power. The instantaneous optical damage threshold was measured as a function of the trap depth as well. These results may help to optimize the optical trapping of biological cells using femtosecond lasers.

AB - Microparticles including biological cells were trapped and manipulated using a continuous wave tweezer and a femtosecond laser tweezer. The difference of the optical trapping force between CW and femtosecond optical tweezers increased as the particle size decreased, possibly due to the self-focusing effect of the ultrashort pulses. Also, the white damage spots were generated near the focus during femtosecond optical trapping of biological cells even with extremely low average power. The instantaneous optical damage threshold was measured as a function of the trap depth as well. These results may help to optimize the optical trapping of biological cells using femtosecond lasers.

KW - Femtosecond lasers

KW - Laser damage

KW - Optical trapping

KW - Tweezers

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U2 - 10.1117/12.591446

DO - 10.1117/12.591446

M3 - Conference article

AN - SCOPUS:21844458427

SN - 1605-7422

VL - 5699

SP - 274

EP - 280

JO - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

JF - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

M1 - 40

T2 - Imaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals and Applications III

Y2 - 24 January 2005 through 27 January 2005

ER -

Cell damage during femtosecond optical trapping

Abstract

Keywords

ASJC Scopus subject areas

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