Cell growth and oxygen transfer in Methylosinus trichosporium OB3b cultures

Jeewon Lee; Bhupendra K. Soni; Robert L. Kelley

doi:10.1007/BF00154618

Cell growth and oxygen transfer in Methylosinus trichosporium OB3b cultures

Jeewon Lee, Bhupendra K. Soni, Robert L. Kelley

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

The steady-state concentration of M. trichosporium OB3b increased about two-fold in the continuous culture when the feeding medium was supplemented by ferrous sulphate (50 mg/L) and citric acid (100 mg/L) at a steady state. In batch and continuous cultures, the cell growth was significantly inhibited by excess N-sources (NH₄0H, NH₄CI, NH₄N0₃, HN0₃, and NaN0₃) and ammonium N-sources were more inhibitory. Both volumetric O₂ transfer coefficient and specific 0₂ uptake rate increased monotonously in an extensive range of air flow rate (0.1-7 vvm) and the methane interfered with the 0₂ transfer even at very low flow rates (0.01-0.1 vvm).

Original language	English
Pages (from-to)	903-908
Number of pages	6
Journal	Biotechnology letters
Volume	18
Issue number	8
DOIs	https://doi.org/10.1007/BF00154618
Publication status	Published - 1996
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1007/BF00154618

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title = "Cell growth and oxygen transfer in Methylosinus trichosporium OB3b cultures",

abstract = "The steady-state concentration of M. trichosporium OB3b increased about two-fold in the continuous culture when the feeding medium was supplemented by ferrous sulphate (50 mg/L) and citric acid (100 mg/L) at a steady state. In batch and continuous cultures, the cell growth was significantly inhibited by excess N-sources (NH40H, NH4CI, NH4N03, HN03, and NaN03) and ammonium N-sources were more inhibitory. Both volumetric O2 transfer coefficient and specific 02 uptake rate increased monotonously in an extensive range of air flow rate (0.1-7 vvm) and the methane interfered with the 02 transfer even at very low flow rates (0.01-0.1 vvm).",

author = "Jeewon Lee and Soni, {Bhupendra K.} and Kelley, {Robert L.}",

year = "1996",

doi = "10.1007/BF00154618",

language = "English",

volume = "18",

pages = "903--908",

journal = "Biotechnology letters",

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publisher = "Springer Science and Business Media B.V.",

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TY - JOUR

T1 - Cell growth and oxygen transfer in Methylosinus trichosporium OB3b cultures

AU - Lee, Jeewon

AU - Soni, Bhupendra K.

AU - Kelley, Robert L.

PY - 1996

Y1 - 1996

N2 - The steady-state concentration of M. trichosporium OB3b increased about two-fold in the continuous culture when the feeding medium was supplemented by ferrous sulphate (50 mg/L) and citric acid (100 mg/L) at a steady state. In batch and continuous cultures, the cell growth was significantly inhibited by excess N-sources (NH40H, NH4CI, NH4N03, HN03, and NaN03) and ammonium N-sources were more inhibitory. Both volumetric O2 transfer coefficient and specific 02 uptake rate increased monotonously in an extensive range of air flow rate (0.1-7 vvm) and the methane interfered with the 02 transfer even at very low flow rates (0.01-0.1 vvm).

AB - The steady-state concentration of M. trichosporium OB3b increased about two-fold in the continuous culture when the feeding medium was supplemented by ferrous sulphate (50 mg/L) and citric acid (100 mg/L) at a steady state. In batch and continuous cultures, the cell growth was significantly inhibited by excess N-sources (NH40H, NH4CI, NH4N03, HN03, and NaN03) and ammonium N-sources were more inhibitory. Both volumetric O2 transfer coefficient and specific 02 uptake rate increased monotonously in an extensive range of air flow rate (0.1-7 vvm) and the methane interfered with the 02 transfer even at very low flow rates (0.01-0.1 vvm).

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U2 - 10.1007/BF00154618

DO - 10.1007/BF00154618

M3 - Article

AN - SCOPUS:0029782202

SN - 0141-5492

VL - 18

SP - 903

EP - 908

JO - Biotechnology letters

JF - Biotechnology letters

IS - 8

ER -

Cell growth and oxygen transfer in Methylosinus trichosporium OB3b cultures

Abstract

ASJC Scopus subject areas

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