Cellular internalization of a membrane binding two-photon probe by a complex of anionic diblock copolymer and cationic surfactant

Okhil Kumar Nag; Han Young Woo; Wei R. Chen

doi:10.1117/12.901836

Cellular internalization of a membrane binding two-photon probe by a complex of anionic diblock copolymer and cationic surfactant

Okhil Kumar Nag, Han Young Woo, Wei R. Chen

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

We report a two-photon (TP) absorbing molecular probe 1,4-bis(4'-(N,N- bis(6''-(N,N,N-trimethylammonium)hexyl)amino)-styryl)benzene tetrabromide (C1) and its interaction with cells upon encapsulation with polymeric vesicles. Two-photon microscopy (TPM) revealed that the free C1 specifically could bind to the plasma membrane and shows bright TP emission. However, C1 encapsulated with polymeric vesicles internalized into the cytosol. In addition, fluorescence quantum efficiency and TP cross section of encapsulated C1 enhanced by 2-fold. These results not only show useful guidelines for the development of efficient TP probes, but also underscore the possibility of using this type of nanostructure for intracellular delivery of the bioactive therapeutics.

Original language	English
Title of host publication	Biophotonics and Immune Responses VII
DOIs	https://doi.org/10.1117/12.901836
Publication status	Published - 2012
Externally published	Yes
Event	Biophotonics and Immune Responses VII - San Francisco, CA, United States Duration: 2012 Jan 23 → 2012 Jan 23

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	8224
ISSN (Print)	1605-7422

Conference

Conference	Biophotonics and Immune Responses VII
Country/Territory	United States
City	San Francisco, CA
Period	12/1/23 → 12/1/23

Keywords

Cellular internalization
Nanocarrier
Polymeric vesicles
Two-photon microscopy

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Biomaterials
Atomic and Molecular Physics, and Optics
Radiology Nuclear Medicine and imaging

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10.1117/12.901836

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Cellular internalization of a membrane binding two-photon probe by a complex of anionic diblock copolymer and cationic surfactant. / Nag, Okhil Kumar; Woo, Han Young; Chen, Wei R.
Biophotonics and Immune Responses VII. 2012. 82240G (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 8224).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Nag, OK, Woo, HY & Chen, WR 2012, Cellular internalization of a membrane binding two-photon probe by a complex of anionic diblock copolymer and cationic surfactant. in Biophotonics and Immune Responses VII., 82240G, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 8224, Biophotonics and Immune Responses VII, San Francisco, CA, United States, 12/1/23. https://doi.org/10.1117/12.901836

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title = "Cellular internalization of a membrane binding two-photon probe by a complex of anionic diblock copolymer and cationic surfactant",

abstract = "We report a two-photon (TP) absorbing molecular probe 1,4-bis(4'-(N,N- bis(6''-(N,N,N-trimethylammonium)hexyl)amino)-styryl)benzene tetrabromide (C1) and its interaction with cells upon encapsulation with polymeric vesicles. Two-photon microscopy (TPM) revealed that the free C1 specifically could bind to the plasma membrane and shows bright TP emission. However, C1 encapsulated with polymeric vesicles internalized into the cytosol. In addition, fluorescence quantum efficiency and TP cross section of encapsulated C1 enhanced by 2-fold. These results not only show useful guidelines for the development of efficient TP probes, but also underscore the possibility of using this type of nanostructure for intracellular delivery of the bioactive therapeutics.",

keywords = "Cellular internalization, Nanocarrier, Polymeric vesicles, Two-photon microscopy",

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T1 - Cellular internalization of a membrane binding two-photon probe by a complex of anionic diblock copolymer and cationic surfactant

AU - Nag, Okhil Kumar

AU - Woo, Han Young

AU - Chen, Wei R.

PY - 2012

Y1 - 2012

N2 - We report a two-photon (TP) absorbing molecular probe 1,4-bis(4'-(N,N- bis(6''-(N,N,N-trimethylammonium)hexyl)amino)-styryl)benzene tetrabromide (C1) and its interaction with cells upon encapsulation with polymeric vesicles. Two-photon microscopy (TPM) revealed that the free C1 specifically could bind to the plasma membrane and shows bright TP emission. However, C1 encapsulated with polymeric vesicles internalized into the cytosol. In addition, fluorescence quantum efficiency and TP cross section of encapsulated C1 enhanced by 2-fold. These results not only show useful guidelines for the development of efficient TP probes, but also underscore the possibility of using this type of nanostructure for intracellular delivery of the bioactive therapeutics.

AB - We report a two-photon (TP) absorbing molecular probe 1,4-bis(4'-(N,N- bis(6''-(N,N,N-trimethylammonium)hexyl)amino)-styryl)benzene tetrabromide (C1) and its interaction with cells upon encapsulation with polymeric vesicles. Two-photon microscopy (TPM) revealed that the free C1 specifically could bind to the plasma membrane and shows bright TP emission. However, C1 encapsulated with polymeric vesicles internalized into the cytosol. In addition, fluorescence quantum efficiency and TP cross section of encapsulated C1 enhanced by 2-fold. These results not only show useful guidelines for the development of efficient TP probes, but also underscore the possibility of using this type of nanostructure for intracellular delivery of the bioactive therapeutics.

KW - Cellular internalization

KW - Nanocarrier

KW - Polymeric vesicles

KW - Two-photon microscopy

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T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

BT - Biophotonics and Immune Responses VII

T2 - Biophotonics and Immune Responses VII

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Cellular internalization of a membrane binding two-photon probe by a complex of anionic diblock copolymer and cationic surfactant

Abstract

Publication series

Conference

Keywords

ASJC Scopus subject areas

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