TY - GEN
T1 - Cellular internalization of a membrane binding two-photon probe by a complex of anionic diblock copolymer and cationic surfactant
AU - Nag, Okhil Kumar
AU - Woo, Han Young
AU - Chen, Wei R.
PY - 2012
Y1 - 2012
N2 - We report a two-photon (TP) absorbing molecular probe 1,4-bis(4'-(N,N- bis(6''-(N,N,N-trimethylammonium)hexyl)amino)-styryl)benzene tetrabromide (C1) and its interaction with cells upon encapsulation with polymeric vesicles. Two-photon microscopy (TPM) revealed that the free C1 specifically could bind to the plasma membrane and shows bright TP emission. However, C1 encapsulated with polymeric vesicles internalized into the cytosol. In addition, fluorescence quantum efficiency and TP cross section of encapsulated C1 enhanced by 2-fold. These results not only show useful guidelines for the development of efficient TP probes, but also underscore the possibility of using this type of nanostructure for intracellular delivery of the bioactive therapeutics.
AB - We report a two-photon (TP) absorbing molecular probe 1,4-bis(4'-(N,N- bis(6''-(N,N,N-trimethylammonium)hexyl)amino)-styryl)benzene tetrabromide (C1) and its interaction with cells upon encapsulation with polymeric vesicles. Two-photon microscopy (TPM) revealed that the free C1 specifically could bind to the plasma membrane and shows bright TP emission. However, C1 encapsulated with polymeric vesicles internalized into the cytosol. In addition, fluorescence quantum efficiency and TP cross section of encapsulated C1 enhanced by 2-fold. These results not only show useful guidelines for the development of efficient TP probes, but also underscore the possibility of using this type of nanostructure for intracellular delivery of the bioactive therapeutics.
KW - Cellular internalization
KW - Nanocarrier
KW - Polymeric vesicles
KW - Two-photon microscopy
UR - http://www.scopus.com/inward/record.url?scp=84859090211&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84859090211&partnerID=8YFLogxK
U2 - 10.1117/12.901836
DO - 10.1117/12.901836
M3 - Conference contribution
AN - SCOPUS:84859090211
SN - 9780819488671
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Biophotonics and Immune Responses VII
T2 - Biophotonics and Immune Responses VII
Y2 - 23 January 2012 through 23 January 2012
ER -