TY - JOUR
T1 - Cellular toxicity of various inhalable metal nanoparticles on human alveolar epithelial cells
AU - Park, Seoyoung
AU - Lee, Yong Kwon
AU - Jung, Moonju
AU - Kim, Ki Heon
AU - Chung, Namhyun
AU - Ahn, Eun Kyung
AU - Lim, Young
AU - Lee, Kweon Haeng
N1 - Funding Information:
Received 16 June 2006; accepted 6 November 2006. This study was supported by the CEFV (Center for Environmentally Friendly Vehicle) of Eco-STAR project from MOE (Ministry of Environment), Republic of Korea. Address correspondence to Prof. Namhyun Chung, Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 136-713, Korea. E-mail: nchung@korea.ac.kr
PY - 2007
Y1 - 2007
N2 - Nanoparticles (NPs) have a greater potential to travel through an organism via inhalation than any other larger particles, and could be more toxic due to their larger surface area and specific structural/chemical properties. The aim of this study was to evaluate in vitro biological effects of various inhalable metallic NPs (TiO2, Ag, Al, Zn, Ni). Human alveolar epithelial cells (A549) were exposed to various concentrations of NPs for 24 h. The extent of morphological damage was in the order of m-TiO2 > n-TiO2 > m-silica >> n-Ni ≈ n-Zn ≈ n-Ag ≈ n-Al and was affected in a dose-dependent manner. The extent of apoptotic damage measured with two-color flow cytometry was in the order of n-Zn > n- Ni > m-silica >> n- TiO2 > m- TiO2 > n-Al > n-Ag. The extent of apoptotic damage measured with DNA fragmentation was in the order of n-Zn ≈ m-silica > n- Ni >> m- TiO2 ≈ n- TiO2 ≈ n-Al > n-Ag, indicating no significant difference in the damages by both m-TiO2 and n-TiO2. The extents of apoptotic damages were also affected in a dose-dependent manner. Uptake of no other NPs but n-TiO2 and m-TiO2 into the cells was observed after 24 h exposure. The intracellular generation of ROS was significant with n-Zn but not with the other particles. These results demonstrated that various inhalable metallic NPs (TiO2, Ag, Al, Zn, Ni) could cause cell damages directly or indirectly. More detailed studies on the influence of size, structure, and composition of the NPs are needed to better understand their toxic mechanisms.
AB - Nanoparticles (NPs) have a greater potential to travel through an organism via inhalation than any other larger particles, and could be more toxic due to their larger surface area and specific structural/chemical properties. The aim of this study was to evaluate in vitro biological effects of various inhalable metallic NPs (TiO2, Ag, Al, Zn, Ni). Human alveolar epithelial cells (A549) were exposed to various concentrations of NPs for 24 h. The extent of morphological damage was in the order of m-TiO2 > n-TiO2 > m-silica >> n-Ni ≈ n-Zn ≈ n-Ag ≈ n-Al and was affected in a dose-dependent manner. The extent of apoptotic damage measured with two-color flow cytometry was in the order of n-Zn > n- Ni > m-silica >> n- TiO2 > m- TiO2 > n-Al > n-Ag. The extent of apoptotic damage measured with DNA fragmentation was in the order of n-Zn ≈ m-silica > n- Ni >> m- TiO2 ≈ n- TiO2 ≈ n-Al > n-Ag, indicating no significant difference in the damages by both m-TiO2 and n-TiO2. The extents of apoptotic damages were also affected in a dose-dependent manner. Uptake of no other NPs but n-TiO2 and m-TiO2 into the cells was observed after 24 h exposure. The intracellular generation of ROS was significant with n-Zn but not with the other particles. These results demonstrated that various inhalable metallic NPs (TiO2, Ag, Al, Zn, Ni) could cause cell damages directly or indirectly. More detailed studies on the influence of size, structure, and composition of the NPs are needed to better understand their toxic mechanisms.
UR - http://www.scopus.com/inward/record.url?scp=34548832559&partnerID=8YFLogxK
U2 - 10.1080/08958370701493282
DO - 10.1080/08958370701493282
M3 - Article
C2 - 17886052
AN - SCOPUS:34548832559
SN - 0895-8378
VL - 19
SP - 59
EP - 65
JO - Inhalation Toxicology
JF - Inhalation Toxicology
IS - SUPPL. 1
ER -