Cellytics: A Digital Inline Holography Platform for Single Cell Analysis in Biomedical and Environmental Applications

In this study, we explore the application of digital inline holography (DIH) and lens-free Shadow imaging technology (LSIT) for biosensing. LSIT offers a cost-effective and rapid method for cell analysis and real-time marine pollution monitoring without complex optical equipment. LSIT provides a cost-effective, rapid method for cell analysis and real-time marine pollution monitoring without complex optical equipment, offering a broad field of view. We analyzed human blood cells using parameters of the shadow images obtained using LSIT, which revealed significant differences and clear group separations. For polymer microspheres, contour algorithms were employed, revealing distinct group characteristics. Furthermore, using machine learning techniques, LSIT effectively tracked rotifer mobility in toxic environments with high accuracy. These results illustrate the potential of LSIT in biosensing, environmental monitoring, and clinical diagnostics.