Central role of Prominin-1 in lipid rafts during liver regeneration

Myeong Suk Bahn, Dong Min Yu, Myoungwoo Lee, Sung Je Jo, Ji Won Lee, Ho Chul Kim, Hyun Lee, Hong Lim Kim, Arum Kim, Jeong Ho Hong, Jun Seok Kim, Seung Hoi Koo, Jae Seon Lee, Young Gyu Ko

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)


Prominin-1, a lipid raft protein, is required for maintaining cancer stem cell properties in hepatocarcinoma cell lines, but its physiological roles in the liver have not been well studied. Here, we investigate the role of Prominin-1 in lipid rafts during liver regeneration and show that expression of Prominin-1 increases after 2/3 partial hepatectomy or CCl4 injection. Hepatocyte proliferation and liver regeneration are attenuated in liver-specific Prominin-1 knockout mice compared to wild-type mice. Detailed mechanistic studies reveal that Prominin-1 interacts with the interleukin-6 signal transducer glycoprotein 130, confining it to lipid rafts so that STAT3 signaling by IL-6 is effectively activated. The overexpression of the glycosylphosphatidylinsositol-anchored first extracellular domain of Prominin-1, which is the domain that binds to GP130, rescued the proliferation of hepatocytes and liver regeneration in liver-specific Prominin-1 knockout mice. In summary, Prominin-1 is upregulated in hepatocytes during liver regeneration where it recruits GP130 into lipid rafts and activates the IL6-GP130-STAT3 axis, suggesting that Prominin-1 might be a promising target for therapeutic applications in liver transplantation.

Original languageEnglish
Article number6219
JournalNature communications
Issue number1
Publication statusPublished - 2022 Dec

Bibliographical note

Funding Information:
This work was supported by grants from the National Research Foundation of Korea awarded to; Y.-G. Ko (R1509597 and R20000552) and J.-S. Lee (R1A5A2031612).

Publisher Copyright:
© 2022, The Author(s).

ASJC Scopus subject areas

  • General Physics and Astronomy
  • General Chemistry
  • General Biochemistry,Genetics and Molecular Biology


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