Changes in locomotor activity and oxidative stress-related factors after the administration of an amino acid mixture by generation and age

Yejin Ahn, Ki Bae Hong, Suhyeon Kim, Hyung Joo Suh, Kyungae Jo

Research output: Contribution to journalArticlepeer-review


Amino acids, as nutrients, are expected to improve sleep disorders. This study aimed to evaluate the generation-and age-dependent sleep-improving effects of γ-aminobutyric acid (GABA) and 5-hydroxytryptophan (5-HTP) coadministration. The differentially expressed genes and generation-related behavior after the administration of a GABA/5-HTP mixture were measured in a Drosophila model, while age-related changes in gene expression and oxidative stress-related parameters were measured in a mouse model. The GABA/5-HTP-treated group showed significant behavioral changes compared to the other groups. Sequencing revealed that the GABA/5-HTP mixture influenced changes in nervous system-related genes, including those involved in the regulation of the expression of behavioral and synaptic genes. Additionally, total sleep time increased with age, and nighttime sleep time in the first-and third-generation flies was significantly different from that of the control groups. The GABA/5-HTP mixture induced significant changes in the expression of sleep-related receptors in both models. Furthermore, the GABA/5-HTP mixture reduced levels of ROS and ROS reaction products in an age-dependent manner. Therefore, the increase in behavioral changes caused by GABA/5-HTP mixture administration was effective in eliminating ROS activity across generations and ages.

Original languageEnglish
Article number9822
JournalInternational journal of molecular sciences
Issue number18
Publication statusPublished - 2021 Sept

Bibliographical note

Funding Information:
Funding: This research was funded by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT, and Future Planning grant number 2014R1A2A1A11052187.

Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.


  • 5-hydroxytryptophan
  • Age
  • Aminobutyric acid
  • Generation
  • Reactive oxygen species
  • Sleep

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry


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