Abstract
BACKGROUND: To isolate polysaccharides with enhanced immunostimulatory activity from Dendrobium officinale, which is used as a herbal medicine in China and Southeast Asia, D. officinale (DO) was pretreated with organic solvents (DOOS) or puffing at 7.5 and 9.0 kgf (7.5DO and 9DO). Hot-water extracts (DOOS-HW, 7.5DO-HW and 9DO-HW) were prepared from each pretreated DO, along with non-pretreated DO, and crude polysaccharides (DO-CP, DOOS-CP, 7.5DO-CP and 9DO-CP) were fractionated from each hot-water extract using ethanol (five volumes). RESULTS: When their immunostimulatory activities were compared by macrophage stimulation and intestinal immune system modulation via Peyer's patches, DOOS-CP showed more potent activity than DO-CP. However, crude polysaccharides fractionated from puffed DO showed significantly lower activity than non-puffed DO and DOOS. The most active polysaccharide contained 95% or more neutral sugar, and the composition ratio of mannose and glucose was 3.0, whereas the lowest polysaccharide content was 2.0 or less. In addition, DOOS-CP was a somewhat refined fraction containing a major peak, representing a molecular weight of 250 kDa, despite being a crude polysaccharide. CONCLUSION: These results suggest that pretreatment of D. officinale with organic solvents may enhance the immunostimulatory activity of polysaccharides and affect the mannose/glucose ratio of polysaccharides, which plays an important role in immunostimulation.
Original language | English |
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Pages (from-to) | 3021-3028 |
Number of pages | 8 |
Journal | Journal of the Science of Food and Agriculture |
Volume | 102 |
Issue number | 7 |
DOIs | |
Publication status | Published - 2022 May |
Bibliographical note
Funding Information:This work was supported by the Priority Research Centers Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Education (2018R1A6A1A03025159).
Publisher Copyright:
© 2021 Society of Chemical Industry.
Keywords
- Dendrobium officinale
- glucomannan
- immunostimulating activity
- physicochemical pretreatment
- polysaccharide
ASJC Scopus subject areas
- Biotechnology
- Food Science
- Agronomy and Crop Science
- Nutrition and Dietetics