Changes in the gut microbiome after galactooligosaccharide administration in loperamideinduced constipation

Min Guk Kim, Kyungae Jo, Yeok Boo Chang, Hyung Joo Suh, Ki Bae Hong

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)


Unbalanced dietary habits and the consumption of high protein and instant foods cause an increase in constipation. Here, we evaluated the effects of galacto-oligosaccharide (GOS) on a rat model of loperamide-induced constipation by measuring various biological markers and cecal microbiota. The fecal water content and intestinal transit ratio significantly increased in the GOSadministered (GL and GH) groups than in the control group (p < 0.05, p < 0.01, and p < 0.001, respectively). The length of intestinal mucosa (p < 0.05 and p < 0.01, respectively) and area of crypt cells were (p < 0.01, both) significantly increased in the GOS-administered groups compared to the control group. The distribution of interstitial cells of Cajal, which is related to the intestinal movement, showed a significant increase in GOS-administered groups than in the control group (p < 0.01, both). The relative abundance of lactic acid bacteria (LAB), especially Lactobacillus and Lactococcus, significantly increased in the GL group than in the control group. Furthermore, there was a positive correlation between short chain fatty acids (SCFAs) and the gut microbiota in the GL groups. These results demonstrated that GOS administration effectively alleviates constipation by increasing LAB proliferation in the intestinal microbiota and SCFA production.

Original languageEnglish
Article number161
Pages (from-to)1-16
Number of pages16
JournalJournal of Personalized Medicine
Issue number4
Publication statusPublished - 2020 Dec

Bibliographical note

Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.


  • Constipation
  • Galacto-oligosaccharide
  • Loperamide
  • Microbiome

ASJC Scopus subject areas

  • Medicine (miscellaneous)


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