Characterization and expression of a presomitic mesoderm-specific mespo gene in zebrafish

Kyeong Won Yoo; Cheol Hee Kim; Hae Chul Park; Seok Hyung Kim; Hyung Seok Kim; Sung Kook Hong; Sangtae Han; Myungchull Rhee; Tae Lin Huh

doi:10.1007/s00427-003-0312-1

Characterization and expression of a presomitic mesoderm-specific mespo gene in zebrafish

Kyeong Won Yoo, Cheol Hee Kim, Hae Chul Park, Seok Hyung Kim, Hyung Seok Kim, Sung Kook Hong, Sangtae Han, Myungchull Rhee, Tae Lin Huh

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

A complete zebrafish mespo cDNA encoding a protein of 131 amino acids with a bHLH domain in the C-terminal has been isolated. The bHLH domain of zebrafish Mespo is highly similar to those in the mouse, chick and Xenopus, sharing 82.4%, 80.4% and 74.5% amino acid identity, respectively. At 50% epiboly, the zebrafish mespo is first detected in the marginal zone of the blastoderm but excluding the prospective shield. Subsequently, mespo expression is intensified in the involuting mesoderm at 60% epiboly, and then restricted to the presomitic mesoderm (PSM) at 95% epiboly. At the 1-somite stage, mespo expression becomes reduced in the most rostral PSM. During segmentation, mespo expression is gradually downregulated at the most rostral segmental plate where cells are being coalesced to form somites. In spadetail mutant embryos, most of mespo-expressing cells were missing.

Original language	English
Pages (from-to)	203-206
Number of pages	4
Journal	Development Genes and Evolution
Volume	213
Issue number	4
DOIs	https://doi.org/10.1007/s00427-003-0312-1
Publication status	Published - 2003 May 1
Externally published	Yes

Bibliographical note

Funding Information:
Acknowledgements This work was supported by grant number FG-4-07 of 21C Frontier Functional Human Genome Project from the Ministry of Science and Technology of Korea.

Keywords

Mespo
Presomitic mesoderm
Spadetail
Zebrafish
bHLH

ASJC Scopus subject areas

Genetics
Developmental Biology

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10.1007/s00427-003-0312-1

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abstract = "A complete zebrafish mespo cDNA encoding a protein of 131 amino acids with a bHLH domain in the C-terminal has been isolated. The bHLH domain of zebrafish Mespo is highly similar to those in the mouse, chick and Xenopus, sharing 82.4%, 80.4% and 74.5% amino acid identity, respectively. At 50% epiboly, the zebrafish mespo is first detected in the marginal zone of the blastoderm but excluding the prospective shield. Subsequently, mespo expression is intensified in the involuting mesoderm at 60% epiboly, and then restricted to the presomitic mesoderm (PSM) at 95% epiboly. At the 1-somite stage, mespo expression becomes reduced in the most rostral PSM. During segmentation, mespo expression is gradually downregulated at the most rostral segmental plate where cells are being coalesced to form somites. In spadetail mutant embryos, most of mespo-expressing cells were missing.",

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AU - Hong, Sung Kook

AU - Han, Sangtae

AU - Rhee, Myungchull

AU - Huh, Tae Lin

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N2 - A complete zebrafish mespo cDNA encoding a protein of 131 amino acids with a bHLH domain in the C-terminal has been isolated. The bHLH domain of zebrafish Mespo is highly similar to those in the mouse, chick and Xenopus, sharing 82.4%, 80.4% and 74.5% amino acid identity, respectively. At 50% epiboly, the zebrafish mespo is first detected in the marginal zone of the blastoderm but excluding the prospective shield. Subsequently, mespo expression is intensified in the involuting mesoderm at 60% epiboly, and then restricted to the presomitic mesoderm (PSM) at 95% epiboly. At the 1-somite stage, mespo expression becomes reduced in the most rostral PSM. During segmentation, mespo expression is gradually downregulated at the most rostral segmental plate where cells are being coalesced to form somites. In spadetail mutant embryos, most of mespo-expressing cells were missing.

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Characterization and expression of a presomitic mesoderm-specific mespo gene in zebrafish

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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