Characterization and immunomodulation activity of Lactobacillus sakei L2 and L8 isolated from chicken cecum

Insuk Sim, Keun Tae Park, Young Hee Lim

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


The aim of this study was to investigate the potential of lactic acid bacteria (LAB) strains as probiotics. Two strains were isolated from healthy chicken cecum and their acid and bile tolerance, residual organic acids, antibacterial activity against pathogenic bacteria, and immunomodulation activity were measured. Identification of the isolated strains was performed using the API 50CHL system and phylogenetic analysis using 16S rDNA sequencing. The isolates were determined to be Lactobacillus sakei strains. The acid tolerance of strains L2 and L8 was high enough that 75% of the inoculum survived in pH 2 for 2 h. The bile tolerance of both strains was observed at a 1% Oxgall concentration in MRS broth. The production of organic acids (lactic acid and acetic acid) and pH changes during growth were monitored and the maximum concentrations were obtained after 48 h of incubation. Culture supernatants of the two LAB strains showed strong antibacterial activity against pathogenic bacteria. The heat-killed LAB cells also induced high levels of immune cell proliferation compared with the control, and stimulated IL-6 and TNF-α production in mouse macrophages. Therefore, L. sakei strains L2 and L8 can be considered suitable probiotic bacteria.

Original languageEnglish
Pages (from-to)201-207
Number of pages7
JournalMicrobiology and Biotechnology Letters
Issue number2
Publication statusPublished - 2016 Jun

Bibliographical note

Publisher Copyright:
© 2016, The Korean Society for Microbiology and Biotechnology.


  • Antibacterial activity
  • Immunomodulation activity
  • Lactobacillus sakei

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology
  • Applied Microbiology and Biotechnology


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