Characterization of a novel mouse brain gene (mbu-1) identified by digital differential display

Hye Lim Yang; Eun Young Cho; Kyung Hee Han; Hyun Kim; Sun Jung Kim

doi:10.1016/j.gene.2007.03.005

Characterization of a novel mouse brain gene (mbu-1) identified by digital differential display

Hye Lim Yang, Eun Young Cho, Kyung Hee Han, Hyun Kim, Sun Jung Kim^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Using in silico approaches, we cloned a novel mouse gene (mbu-1) that was strictly expressed in the central nervous system. mbu-1 was first identified as an EST after carrying out digital differential display for unigene libraries from various mouse tissues. The full-length cDNA sequence was obtained by extending the ends of EST by RACE. The cDNA sequence was 2611 bp long and contained an ORF of 597 AA. A positive cis-acting region was found in the neuroblastoma × glioma hybrid, NG108-15, and in human embryonic kidney HEK293 cell lines. RT-PCR and in situ hybridization analysis showed that the mbu-1 gene was only expressed in the brain and spinal cord during the embryonic stages, and throughout all regions of the adult brain, showing higher levels in the hippocampus and hypothalamus.

Original language	English
Pages (from-to)	144-150
Number of pages	7
Journal	Gene
Volume	395
Issue number	1-2
DOIs	https://doi.org/10.1016/j.gene.2007.03.005
Publication status	Published - 2007 Jun 15

Bibliographical note

Funding Information:
This work was supported by a research fund from Dongguk University (2006–2009-0).

Keywords

In silico cloning
In situ hybridization
NG108-15
Promoter
Unigene

ASJC Scopus subject areas

Genetics

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10.1016/j.gene.2007.03.005

Cite this

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title = "Characterization of a novel mouse brain gene (mbu-1) identified by digital differential display",

abstract = "Using in silico approaches, we cloned a novel mouse gene (mbu-1) that was strictly expressed in the central nervous system. mbu-1 was first identified as an EST after carrying out digital differential display for unigene libraries from various mouse tissues. The full-length cDNA sequence was obtained by extending the ends of EST by RACE. The cDNA sequence was 2611 bp long and contained an ORF of 597 AA. A positive cis-acting region was found in the neuroblastoma × glioma hybrid, NG108-15, and in human embryonic kidney HEK293 cell lines. RT-PCR and in situ hybridization analysis showed that the mbu-1 gene was only expressed in the brain and spinal cord during the embryonic stages, and throughout all regions of the adult brain, showing higher levels in the hippocampus and hypothalamus.",

keywords = "In silico cloning, In situ hybridization, NG108-15, Promoter, Unigene",

author = "Yang, \{Hye Lim\} and Cho, \{Eun Young\} and Han, \{Kyung Hee\} and Hyun Kim and Kim, \{Sun Jung\}",

note = "Funding Information: This work was supported by a research fund from Dongguk University (2006–2009-0).",

year = "2007",

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doi = "10.1016/j.gene.2007.03.005",

language = "English",

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AU - Yang, Hye Lim

AU - Cho, Eun Young

AU - Han, Kyung Hee

AU - Kim, Hyun

AU - Kim, Sun Jung

N1 - Funding Information: This work was supported by a research fund from Dongguk University (2006–2009-0).

PY - 2007/6/15

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AB - Using in silico approaches, we cloned a novel mouse gene (mbu-1) that was strictly expressed in the central nervous system. mbu-1 was first identified as an EST after carrying out digital differential display for unigene libraries from various mouse tissues. The full-length cDNA sequence was obtained by extending the ends of EST by RACE. The cDNA sequence was 2611 bp long and contained an ORF of 597 AA. A positive cis-acting region was found in the neuroblastoma × glioma hybrid, NG108-15, and in human embryonic kidney HEK293 cell lines. RT-PCR and in situ hybridization analysis showed that the mbu-1 gene was only expressed in the brain and spinal cord during the embryonic stages, and throughout all regions of the adult brain, showing higher levels in the hippocampus and hypothalamus.

KW - In silico cloning

KW - In situ hybridization

KW - NG108-15

KW - Promoter

KW - Unigene

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DO - 10.1016/j.gene.2007.03.005

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AN - SCOPUS:34247609705

SN - 0378-1119

VL - 395

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EP - 150

JO - Gene

JF - Gene

IS - 1-2

ER -

Characterization of a novel mouse brain gene (mbu-1) identified by digital differential display

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

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