Here, we report the cloning and characterization of a common salivary gland-specific gene, Nf-1, from late instar Hydropsyche sp. larvae, and show that the corresponding gene product is translated in the gland and secreted to the gland lumen. The deduced Nf-1 protein is primarily composed of five repetitive sequence units of 63-65 amino acids, and contains a putative signal sequence composed of 19 amino acids. Secreted Nf-1 (∼37 kDa) was localized to the gland lumen by Western blotting of gland and lumen fractions. Together, the structure, expression pattern and protein localization of Nf-1 indicate that this protein is likely to be a major component of the silk shields and nets produced by the aquatic insect, Trichoptera.
Bibliographical noteFunding Information:
This study was supported by the Bio-Green 21 Grant from the Rural Development Administration of Korea and the Brain Korea 21 Grant from the Ministry of Education of Korea to S.S. Han in 2004.
- Expressed sequence tags
- Modified salivary gland
- Nf-1 protein (Nest forming protein-1)
- Repetitive amino acid
- Trichoptera Hydropsyche sp.
ASJC Scopus subject areas
- Molecular Biology
- Insect Science