Characterization of a novel repetitive secretory protein specifically expressed in the modified salivary gland of Hydropsyche sp. (Trichoptera; Hydropsychidae)

Jai Hoon Eum; Sung Moon Yoe; Young R. Seo; Seok Woo Kang; Sung Sik Han

doi:10.1016/j.ibmb.2005.01.009

Characterization of a novel repetitive secretory protein specifically expressed in the modified salivary gland of Hydropsyche sp. (Trichoptera; Hydropsychidae)

Jai Hoon Eum, Sung Moon Yoe, Young R. Seo, Seok Woo Kang, Sung Sik Han

* Honorary professors

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Here, we report the cloning and characterization of a common salivary gland-specific gene, Nf-1, from late instar Hydropsyche sp. larvae, and show that the corresponding gene product is translated in the gland and secreted to the gland lumen. The deduced Nf-1 protein is primarily composed of five repetitive sequence units of 63-65 amino acids, and contains a putative signal sequence composed of 19 amino acids. Secreted Nf-1 (∼37 kDa) was localized to the gland lumen by Western blotting of gland and lumen fractions. Together, the structure, expression pattern and protein localization of Nf-1 indicate that this protein is likely to be a major component of the silk shields and nets produced by the aquatic insect, Trichoptera.

Original language	English
Pages (from-to)	435-441
Number of pages	7
Journal	Insect Biochemistry and Molecular Biology
Volume	35
Issue number	5
DOIs	https://doi.org/10.1016/j.ibmb.2005.01.009
Publication status	Published - 2005 May

Bibliographical note

Funding Information:
This study was supported by the Bio-Green 21 Grant from the Rural Development Administration of Korea and the Brain Korea 21 Grant from the Ministry of Education of Korea to S.S. Han in 2004.

Keywords

Expressed sequence tags
Modified salivary gland
Nf-1 protein (Nest forming protein-1)
Repetitive amino acid
Trichoptera Hydropsyche sp.

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Insect Science

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10.1016/j.ibmb.2005.01.009

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title = "Characterization of a novel repetitive secretory protein specifically expressed in the modified salivary gland of Hydropsyche sp. (Trichoptera; Hydropsychidae)",

abstract = "Here, we report the cloning and characterization of a common salivary gland-specific gene, Nf-1, from late instar Hydropsyche sp. larvae, and show that the corresponding gene product is translated in the gland and secreted to the gland lumen. The deduced Nf-1 protein is primarily composed of five repetitive sequence units of 63-65 amino acids, and contains a putative signal sequence composed of 19 amino acids. Secreted Nf-1 (∼37 kDa) was localized to the gland lumen by Western blotting of gland and lumen fractions. Together, the structure, expression pattern and protein localization of Nf-1 indicate that this protein is likely to be a major component of the silk shields and nets produced by the aquatic insect, Trichoptera.",

keywords = "Expressed sequence tags, Modified salivary gland, Nf-1 protein (Nest forming protein-1), Repetitive amino acid, Trichoptera Hydropsyche sp.",

author = "Eum, {Jai Hoon} and Yoe, {Sung Moon} and Seo, {Young R.} and Kang, {Seok Woo} and Han, {Sung Sik}",

note = "Funding Information: This study was supported by the Bio-Green 21 Grant from the Rural Development Administration of Korea and the Brain Korea 21 Grant from the Ministry of Education of Korea to S.S. Han in 2004.",

year = "2005",

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doi = "10.1016/j.ibmb.2005.01.009",

language = "English",

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AU - Eum, Jai Hoon

AU - Yoe, Sung Moon

AU - Seo, Young R.

AU - Kang, Seok Woo

AU - Han, Sung Sik

N1 - Funding Information: This study was supported by the Bio-Green 21 Grant from the Rural Development Administration of Korea and the Brain Korea 21 Grant from the Ministry of Education of Korea to S.S. Han in 2004.

PY - 2005/5

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N2 - Here, we report the cloning and characterization of a common salivary gland-specific gene, Nf-1, from late instar Hydropsyche sp. larvae, and show that the corresponding gene product is translated in the gland and secreted to the gland lumen. The deduced Nf-1 protein is primarily composed of five repetitive sequence units of 63-65 amino acids, and contains a putative signal sequence composed of 19 amino acids. Secreted Nf-1 (∼37 kDa) was localized to the gland lumen by Western blotting of gland and lumen fractions. Together, the structure, expression pattern and protein localization of Nf-1 indicate that this protein is likely to be a major component of the silk shields and nets produced by the aquatic insect, Trichoptera.

AB - Here, we report the cloning and characterization of a common salivary gland-specific gene, Nf-1, from late instar Hydropsyche sp. larvae, and show that the corresponding gene product is translated in the gland and secreted to the gland lumen. The deduced Nf-1 protein is primarily composed of five repetitive sequence units of 63-65 amino acids, and contains a putative signal sequence composed of 19 amino acids. Secreted Nf-1 (∼37 kDa) was localized to the gland lumen by Western blotting of gland and lumen fractions. Together, the structure, expression pattern and protein localization of Nf-1 indicate that this protein is likely to be a major component of the silk shields and nets produced by the aquatic insect, Trichoptera.

KW - Expressed sequence tags

KW - Modified salivary gland

KW - Nf-1 protein (Nest forming protein-1)

KW - Repetitive amino acid

KW - Trichoptera Hydropsyche sp.

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DO - 10.1016/j.ibmb.2005.01.009

M3 - Article

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AN - SCOPUS:15944399104

SN - 0965-1748

VL - 35

SP - 435

EP - 441

JO - Insect Biochemistry and Molecular Biology

JF - Insect Biochemistry and Molecular Biology

IS - 5

ER -

Characterization of a novel repetitive secretory protein specifically expressed in the modified salivary gland of Hydropsyche sp. (Trichoptera; Hydropsychidae)

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

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