Abstract
Enhanced catalytic activities of different lignocellulases were obtained from Armillaria gemina under statistically optimized parameters using a jar fermenter. This strain showed maximum xylanase, endoglucanase, cellobiohydrolase, and β-glucosidase activities of 1,270, 146, 34, and 15 U mL-1, respectively. Purified A. gemina xylanase (AgXyl) has the highest catalytic efficiency (k cat/K m = 1,440 mg mL -1 s-1) ever reported for any fungal xylanase, highlighting the significance of the current study. We covalently immobilized the crude xylanase preparation onto functionalized silicon oxide nanoparticles, achieving 117 % immobilization efficiency. Further immobilization caused a shift in the optimal pH and temperature, along with a fourfold improvement in the half-life of crude AgXyl. Immobilized AgXyl gave 37.8 % higher production of xylooligosaccharides compared to free enzyme. After 17 cycles, the immobilized enzyme retained 92 % of the original activity, demonstrating its potential for the synthesis of xylooligosaccharides in industrial applications.
Original language | English |
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Pages (from-to) | 1081-1091 |
Number of pages | 11 |
Journal | Applied Microbiology and Biotechnology |
Volume | 97 |
Issue number | 3 |
DOIs | |
Publication status | Published - 2013 Feb |
Externally published | Yes |
Bibliographical note
Funding Information:Acknowledgments This research was supported by the Converging Research Center Program through the National Research Foundation of Korea, funded by the Ministry of Education, Science and Technology (grant 2011–50210). This study was also supported by Brain Pool 2011 of Konkuk University, Republic of Korea.
Keywords
- Denaturation constant
- Enthalpy
- Immobilization
- SiO
- Xylanase
- Xylooligosaccharide
ASJC Scopus subject areas
- Biotechnology
- Applied Microbiology and Biotechnology