Characterization of a novel xylanase from Armillaria gemina and its immobilization onto SiO2 nanoparticles

Saurabh Sudha Dhiman, Dayanand Kalyani, Sujit Sadashiv Jagtap, Jung Rim Haw, Yun Chan Kang, Jung Kul Lee

Research output: Contribution to journalArticlepeer-review

31 Citations (Scopus)

Abstract

Enhanced catalytic activities of different lignocellulases were obtained from Armillaria gemina under statistically optimized parameters using a jar fermenter. This strain showed maximum xylanase, endoglucanase, cellobiohydrolase, and β-glucosidase activities of 1,270, 146, 34, and 15 U mL-1, respectively. Purified A. gemina xylanase (AgXyl) has the highest catalytic efficiency (k cat/K m = 1,440 mg mL -1 s-1) ever reported for any fungal xylanase, highlighting the significance of the current study. We covalently immobilized the crude xylanase preparation onto functionalized silicon oxide nanoparticles, achieving 117 % immobilization efficiency. Further immobilization caused a shift in the optimal pH and temperature, along with a fourfold improvement in the half-life of crude AgXyl. Immobilized AgXyl gave 37.8 % higher production of xylooligosaccharides compared to free enzyme. After 17 cycles, the immobilized enzyme retained 92 % of the original activity, demonstrating its potential for the synthesis of xylooligosaccharides in industrial applications.

Original languageEnglish
Pages (from-to)1081-1091
Number of pages11
JournalApplied Microbiology and Biotechnology
Volume97
Issue number3
DOIs
Publication statusPublished - 2013 Feb
Externally publishedYes

Bibliographical note

Funding Information:
Acknowledgments This research was supported by the Converging Research Center Program through the National Research Foundation of Korea, funded by the Ministry of Education, Science and Technology (grant 2011–50210). This study was also supported by Brain Pool 2011 of Konkuk University, Republic of Korea.

Keywords

  • Denaturation constant
  • Enthalpy
  • Immobilization
  • SiO
  • Xylanase
  • Xylooligosaccharide

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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