TY - JOUR
T1 - Characterization of a recombinant endo-type alginate lyase (Alg7D) from Saccharophagus degradans
AU - Kim, Hee Taek
AU - Ko, Hyeok Jin
AU - Kim, Nahyun
AU - Kim, Duwoon
AU - Lee, Dongho
AU - Choi, In Geol
AU - Woo, Hee Chul
AU - Kim, Myoung Dong
AU - Kim, Kyoung Heon
N1 - Copyright:
Copyright 2012 Elsevier B.V., All rights reserved.
PY - 2012/6
Y1 - 2012/6
N2 - A gene, alg7D, from Saccharophagus degradans, coding for a putative alginate lyase belonging to the family of polysaccharide lyase-7, was overexpressed in Escherichia coli. The properties of the recombinant Alg7D were characterized. The enzyme endolytically depolymerized alginate by β-elimination into oligo-alginates with degrees of polymerization of 2-5. Its activity was maximal at 50°C and pH 7 and was slightly increased in the presence of Na +. The K M, V max, k cat, and k cat/K M values were: 3 mg ml -1, 6.2 U mg -1, 1.9 × 10 -2 s -1, and 6.3 × 10 -3 mg -1ml s -1, respectively.
AB - A gene, alg7D, from Saccharophagus degradans, coding for a putative alginate lyase belonging to the family of polysaccharide lyase-7, was overexpressed in Escherichia coli. The properties of the recombinant Alg7D were characterized. The enzyme endolytically depolymerized alginate by β-elimination into oligo-alginates with degrees of polymerization of 2-5. Its activity was maximal at 50°C and pH 7 and was slightly increased in the presence of Na +. The K M, V max, k cat, and k cat/K M values were: 3 mg ml -1, 6.2 U mg -1, 1.9 × 10 -2 s -1, and 6.3 × 10 -3 mg -1ml s -1, respectively.
KW - Alg7d
KW - Endo-type alginate lyase
KW - Polysaccharide lyase-7
KW - Saccharophagus degradans
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U2 - 10.1007/s10529-012-0876-9
DO - 10.1007/s10529-012-0876-9
M3 - Article
C2 - 22391735
AN - SCOPUS:84862785475
SN - 0141-5492
VL - 34
SP - 1087
EP - 1092
JO - Biotechnology Letters
JF - Biotechnology Letters
IS - 6
ER -