Characterization of a recombinant endo-type alginate lyase (Alg7D) from Saccharophagus degradans

Hee Taek Kim; Hyeok Jin Ko; Nahyun Kim; Duwoon Kim; Dongho Lee; In Geol Choi; Hee Chul Woo; Myoung Dong Kim; Kyoung Heon Kim

doi:10.1007/s10529-012-0876-9

Characterization of a recombinant endo-type alginate lyase (Alg7D) from Saccharophagus degradans

Hee Taek Kim
, Hyeok Jin Ko
, Nahyun Kim
, Duwoon Kim
, Dongho Lee
, In Geol Choi
, Hee Chul Woo
, Myoung Dong Kim
, Kyoung Heon Kim

Research output: Contribution to journal › Article › peer-review

64 Citations (Scopus)

Abstract

A gene, alg7D, from Saccharophagus degradans, coding for a putative alginate lyase belonging to the family of polysaccharide lyase-7, was overexpressed in Escherichia coli. The properties of the recombinant Alg7D were characterized. The enzyme endolytically depolymerized alginate by β-elimination into oligo-alginates with degrees of polymerization of 2-5. Its activity was maximal at 50°C and pH 7 and was slightly increased in the presence of Na ⁺. The K _M, V _max, k _cat, and k _cat/K _M values were: 3 mg ml ^-1, 6.2 U mg ^-1, 1.9 × 10 ^-2 s ^-1, and 6.3 × 10 ^-3 mg ^-1ml s ^-1, respectively.

Original language	English
Pages (from-to)	1087-1092
Number of pages	6
Journal	Biotechnology letters
Volume	34
Issue number	6
DOIs	https://doi.org/10.1007/s10529-012-0876-9
Publication status	Published - 2012 Jun

Bibliographical note

Copyright:
Copyright 2012 Elsevier B.V., All rights reserved.

Keywords

Alg7d
Endo-type alginate lyase
Polysaccharide lyase-7
Saccharophagus degradans

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1007/s10529-012-0876-9

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title = "Characterization of a recombinant endo-type alginate lyase (Alg7D) from Saccharophagus degradans",

abstract = "A gene, alg7D, from Saccharophagus degradans, coding for a putative alginate lyase belonging to the family of polysaccharide lyase-7, was overexpressed in Escherichia coli. The properties of the recombinant Alg7D were characterized. The enzyme endolytically depolymerized alginate by β-elimination into oligo-alginates with degrees of polymerization of 2-5. Its activity was maximal at 50°C and pH 7 and was slightly increased in the presence of Na +. The K M, V max, k cat, and k cat/K M values were: 3 mg ml -1, 6.2 U mg -1, 1.9 × 10 -2 s -1, and 6.3 × 10 -3 mg -1ml s -1, respectively.",

keywords = "Alg7d, Endo-type alginate lyase, Polysaccharide lyase-7, Saccharophagus degradans",

author = "Kim, \{Hee Taek\} and Ko, \{Hyeok Jin\} and Nahyun Kim and Duwoon Kim and Dongho Lee and Choi, \{In Geol\} and Woo, \{Hee Chul\} and Kim, \{Myoung Dong\} and Kim, \{Kyoung Heon\}",

year = "2012",

month = jun,

doi = "10.1007/s10529-012-0876-9",

language = "English",

volume = "34",

pages = "1087--1092",

journal = "Biotechnology letters",

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T1 - Characterization of a recombinant endo-type alginate lyase (Alg7D) from Saccharophagus degradans

AU - Kim, Hee Taek

AU - Ko, Hyeok Jin

AU - Kim, Nahyun

AU - Kim, Duwoon

AU - Lee, Dongho

AU - Choi, In Geol

AU - Woo, Hee Chul

AU - Kim, Myoung Dong

AU - Kim, Kyoung Heon

PY - 2012/6

Y1 - 2012/6

N2 - A gene, alg7D, from Saccharophagus degradans, coding for a putative alginate lyase belonging to the family of polysaccharide lyase-7, was overexpressed in Escherichia coli. The properties of the recombinant Alg7D were characterized. The enzyme endolytically depolymerized alginate by β-elimination into oligo-alginates with degrees of polymerization of 2-5. Its activity was maximal at 50°C and pH 7 and was slightly increased in the presence of Na +. The K M, V max, k cat, and k cat/K M values were: 3 mg ml -1, 6.2 U mg -1, 1.9 × 10 -2 s -1, and 6.3 × 10 -3 mg -1ml s -1, respectively.

AB - A gene, alg7D, from Saccharophagus degradans, coding for a putative alginate lyase belonging to the family of polysaccharide lyase-7, was overexpressed in Escherichia coli. The properties of the recombinant Alg7D were characterized. The enzyme endolytically depolymerized alginate by β-elimination into oligo-alginates with degrees of polymerization of 2-5. Its activity was maximal at 50°C and pH 7 and was slightly increased in the presence of Na +. The K M, V max, k cat, and k cat/K M values were: 3 mg ml -1, 6.2 U mg -1, 1.9 × 10 -2 s -1, and 6.3 × 10 -3 mg -1ml s -1, respectively.

KW - Alg7d

KW - Endo-type alginate lyase

KW - Polysaccharide lyase-7

KW - Saccharophagus degradans

UR - https://www.scopus.com/pages/publications/84862785475

UR - https://www.scopus.com/pages/publications/84862785475#tab=citedBy

U2 - 10.1007/s10529-012-0876-9

DO - 10.1007/s10529-012-0876-9

M3 - Article

C2 - 22391735

AN - SCOPUS:84862785475

SN - 0141-5492

VL - 34

SP - 1087

EP - 1092

JO - Biotechnology letters

JF - Biotechnology letters

IS - 6

ER -

Characterization of a recombinant endo-type alginate lyase (Alg7D) from Saccharophagus degradans

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

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